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Mouse submandibular gland (SMG) acinar cells exhibited a robust regulatory volume decrease (RVD) following cell swelling that was reduced approximately 70% in <jats:italic>Ano6<\/jats:italic><jats:sup><jats:italic>\u2013\/\u2013<\/jats:italic><\/jats:sup> mice. Ca<jats:sup>2+<\/jats:sup>\u2010free conditions nearly eliminated the RVD response suggesting that Ano6 is an obligatory component of the\u00a0cell volume\u2010activated, Ca<jats:sup>2+<\/jats:sup>\u2010dependent RVD pathway in salivary gland acinar cells. <jats:italic>Ex vivo<\/jats:italic> agonist\u2010stimulated secretion of water and ions was unaffected by <jats:italic>Ano6<\/jats:italic> disruption under both isotonic and hypotonic conditions suggesting that Ano6 does not play a major role in fluid and electrolyte secretion. In contrast, the total amount of \u03b2\u2010adrenergic\u2010dependent protein secretion by the SMG was significantly reduced in <jats:italic>Ano6<\/jats:italic><jats:sup><jats:italic>\u2013\/\u2013<\/jats:italic><\/jats:sup> mice. Closer inspection of these latter results revealed that protein secretion was affected only in the female SMG by <jats:italic>Ano6<\/jats:italic> disruption. These results indicate that Ano6 modulates the RVD response and protein secretion by salivary gland acinar cells.<\/jats:p>","DOI":"10.1002\/jcp.29697","type":"journal-article","created":{"date-parts":[[2020,4,24]],"date-time":"2020-04-24T07:34:28Z","timestamp":1587713668000},"page":"8533-8545","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>Ano6<\/i> disruption impairs acinar cell regulatory volume decrease and protein secretion in murine submandibular salivary glands"],"prefix":"10.1002","volume":"235","author":[{"given":"Takashi","family":"Munemasa","sequence":"first","affiliation":[{"name":"Secretory Mechanisms and Dysfunctions Section National Institute of Dental and Craniofacial Research, National Institutes of Health Bethesda Maryland"},{"name":"Division of Oral Reconstruction and Rehabilitation Kyushu Dental University, Kitakyushu Fukuoka 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