{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:27:41Z","timestamp":1788992861234,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T00:00:00Z","timestamp":1589241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371109"],"award-info":[{"award-number":["81371109"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670953"],"award-info":[{"award-number":["81670953"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We wished to evaluate whether epigenetic modifiers have a beneficial effect on treating experimental periodontitis\u00a0and mechanisms for\u00a0regulating the cell fate of mesenchymal stem cells (MSCs) in inflammatory microenvironments. We isolated MSCs from healthy and inflamed gingival tissues to investigate\u00a0whether trichostatin A (TSA) could improve osteogenic differentiation and resolve inflammation in vitro. The tissue regenerative potentials were evaluated when treated with a temperature\u2010dependent, chitosan\u2010scaffold\u2010encapsulated TSA, in a rat model of periodontitis. After induction with the conditioned medium, TSA treatment increased the osteogenic differentiation potential of inflamed MSCs and healthy MSCs. In addition, interleukin\u20106 and interleukin\u20108 levels in supernatants were significantly decreased after TSA treatment. Moreover, TSA promoted osteogenic differentiation by inhibiting nuclear factor\u2010\u03baB (p65) DNA binding in MSCs. In rats with experimental periodontitis, 7 weeks after local injections of chitosan\u2010scaffold\u2010encapsulated TSA, histology and microcomputed tomography showed a significant increase in alveolar bone volume and less inflammatory infiltration compared with vehicle\u2010treated rats. The concentrations of interferon\u2010\u03b3 and interleukin\u20106 were significantly decreased in the gingival crevicular fluid after TSA treatment. This study demonstrated that TSA had anti\u2010inflammatory properties and could promote periodontal tissue repair, which indicated that epigenetic modifiers hold promise as a potential therapeutic option for periodontal tissue repair.<\/jats:p>","DOI":"10.1002\/jcp.29780","type":"journal-article","created":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T05:36:52Z","timestamp":1589348212000},"page":"9691-9701","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Epigenetic modifier trichostatin A enhanced osteogenic differentiation of mesenchymal stem cells by inhibiting NF\u2010\u03baB (p65) DNA binding and promoted periodontal repair in rats"],"prefix":"10.1002","volume":"235","author":[{"given":"Qiong","family":"Li","sequence":"first","affiliation":[{"name":"Department of Endodontics &amp; Laboratory for Dental Stem Cells and Endocrine Immunology Tianjin Medical University School of Stomatology  Tianjin 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