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Inflammatory responses are beneficial in the fetomaternal interface and supposedly accelerate the chances for successful implantation. The current study aims to determine the effect of Toll\u2010like receptor 4 (TLR4) overexpression in mouse blastocysts via Let\u20107a downregulation using intracytoplasmic sperm injection\u2010sperm\u2010mediated gene transfer on embryo attachment rate. The pLenti\u2010III\u2010GFP\u2010miR\u2010Off\u2010Let\u20107a vector was transmitted to oocytes derived via in vitro maturation (IVM) and in vivo oocytes by using NaOH\u2010treated spermatozoa. Let\u20107a and TLR4 expression levels were evaluated by quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR), immunocytochemistry, and western blot analysis in both oocytes and embryos. Blastocyst adhesion on the endometrial cells was monitored by microscopic analysis. qRT\u2010PCR results showed that Let\u20107a expression decreased in the IVM (GV\u2010MII) oocytes compared to the in vivo oocyte (MII) group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). TLR4 showed a higher expression in GV\u2010MII oocytes at both the gene and protein levels (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Following anti\u2010miR\u2010Let\u20107a transmission, the TLR4 expression level was significantly upregulated in embryos compared with the control groups (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Attachment and migration of trophoblasts cells towards endometrial cells dramatically increased compared to the control group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Based on our results, we concluded that Let\u20107a might mediate embryo attachment through regulation of TLR4 expression levels.<\/jats:p>","DOI":"10.1002\/jcp.29787","type":"journal-article","created":{"date-parts":[[2020,5,16]],"date-time":"2020-05-16T13:48:43Z","timestamp":1589636923000},"page":"9752-9762","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Upregulation of Toll\u2010like receptor 4 through anti\u2010miR\u2010Let\u20107a enhances blastocyst attachment to endometrial cells in mice"],"prefix":"10.1002","volume":"235","author":[{"given":"Sara","family":"Hosseini","sequence":"first","affiliation":[{"name":"Cellular and Molecular Biology Research Center Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samaneh","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Department of Stem Cells and Developmental Biology, 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