{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T01:35:04Z","timestamp":1780536904640,"version":"3.54.1"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:00:00Z","timestamp":1589500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81600461"],"award-info":[{"award-number":["81600461"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nonalcoholic fatty liver disease (NAFLD) is regarded as a threat to public health; however, the pathologic mechanism of NAFLD is not fully understood. We attempted to identify abnormally expressed long noncoding RNA (lncRNAs) and messenger RNA that may affect the occurrence and development of NAFLD in this study. The expression of differentially expressed lncRNAs in NAFLD was determined in oleic acid (OA)\u2010treated L02 cells, and the functions of CCAT1 in lipid droplet formation were evaluated in vitro. Differentially expressed genes (DEGs) were analyzed by microarray analysis, and DEGs related to CCTA1 were selected and verified by weighted correlation network analysis. The dynamic effects of <jats:italic>LXR\u03b1<\/jats:italic> and CCTA1 on lipid droplet formation and predicted binding was examined. The binding between miR\u2010631 and CCAT1 and LXR\u03b1 was verified. The dynamic effects of miR\u2010613 inhibition and CCTA1 silencing on lipid droplet formation were examined. The expression and correlations of miR\u2010631, CCAT1, and LXR\u03b1 were determined in tissue samples. As the results show, CCAT1 was induced by OA and upregulated in NAFLD clinical samples. CCAT1 silencing significantly suppressed lipid droplet accumulation in vitro. LXR\u03b1 was positively correlated with CCAT1. By inhibiting miR\u2010613, CCAT1 increased the transcription of LXR\u03b1 and promoted LXR\u03b1 expression. The expression of LXR\u03b1 was significantly increased in NAFLD tissues and was positively correlated with CCAT1. In conclusion, CCAT1 increases LXR\u03b1 transcription by serving as a competing endogenous RNA for miR\u2010613 in an LXRE\u2010dependent manner, thereby promoting lipid droplet formation and NAFLD. CCAT1 and LXR\u03b1 might be potent targets for NAFLD treatment.<\/jats:p>","DOI":"10.1002\/jcp.29795","type":"journal-article","created":{"date-parts":[[2020,5,16]],"date-time":"2020-05-16T06:30:33Z","timestamp":1589610633000},"page":"9819-9833","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Long noncoding RNA CCAT1 inhibits miR\u2010613 to promote nonalcoholic fatty liver disease via increasing <i>LXR\u03b1<\/i> transcription"],"prefix":"10.1002","volume":"235","author":[{"given":"Feizhou","family":"Huang","sequence":"first","affiliation":[{"name":"Emergency Department, The Third Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaizheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Emergency Department, The Third Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Lei","sequence":"additional","affiliation":[{"name":"Emergency Department, The Third Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhou","family":"Li","sequence":"additional","affiliation":[{"name":"Emergency Department, The Third Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Emergency Department, The Third Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingshi","family":"Yang","sequence":"additional","affiliation":[{"name":"Emergency Department, The Third Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kefu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Emergency Department, The Third Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2116-0233","authenticated-orcid":false,"given":"Chuanzheng","family":"Sun","sequence":"additional","affiliation":[{"name":"Emergency Department, The Third Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620\u2010014\u20103289\u2010x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12029\u2010018\u20100060\u20100"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067\u2010016\u20103403\u2010x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.022491"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1139\/bcb\u20102017\u20100154"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1204265"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19102980"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046\u2010015\u20100136\u20107"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1010428317697572"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcp158"},{"issue":"11","key":"e_1_2_9_12_1","first-page":"1058","article-title":"The relationship between resistin and ghrelin levels with fibrosis in nonalcoholic fatty liver disease","volume":"19","author":"Eminler A. 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