{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T19:20:33Z","timestamp":1762543233263,"version":"3.37.3"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,6,14]],"date-time":"2020-06-14T00:00:00Z","timestamp":1592092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004366","name":"Tabriz University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In vitro maturation (IVM) is a novel approach to overcome the adverse effects of human in vitro fertilization (IVF). The aim of the present study is to evaluate the effect of total and steroid\u2010depleted serum obtained from patients with endometriosis on IVM outcome as supplementation for this system. To this purpose, patients with endometriosis were selected according to in\/excluding criteria. Germinal vesicles (GVs) and cumulus cells were treated with 10% of each serum. The expression levels of stearoyl CoA desaturase 1 (SCD 1) and cyclooxygenase\u20102 (COX\u20102) genes were evaluated by RT\u2010qPCR. Gas\u2010liquid chromatography and flow cytometry were performed to analyze fatty acids composition and apoptosis. The mRNA expression levels of SCD1 (2.47 fold) and COX\u20102 (6.4 fold), and also the synthesis of oleate, linoleate, and arachidonate were increased (1.19, 1.06, and 2.37 folds, respectively) in cumulus cells treated with steroid\u2010depleted serum (<jats:italic>p<\/jats:italic> &lt; .05). The synthesis of palmitate, palmitoleate, and stearate (0.995, 0.67, and 0.7 folds, respectively) and also the rate of apoptosis were significantly decreased in these cells (<jats:italic>p<\/jats:italic> &lt; .05). Moreover, GVs cultured in steroid\u2010depleted group showed a significantly higher rate of maturation (<jats:italic>p<\/jats:italic> &lt; .001). Overall, our findings imply a new insight into the expansion of IVM system in oocytes development.<\/jats:p>","DOI":"10.1002\/jcp.29834","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T03:45:04Z","timestamp":1592192704000},"page":"205-214","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Steroid\u2010depleted endometriosis serum improves oocyte maturation in IVM systems"],"prefix":"10.1002","volume":"236","author":[{"given":"Samira","family":"Asghari","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Students Research Center Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5367-9956","authenticated-orcid":false,"given":"Mohammad","family":"Nouri","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6380-272X","authenticated-orcid":false,"given":"Masoud","family":"Darabi","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7852-8118","authenticated-orcid":false,"given":"Amir","family":"Valizadeh","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center Stem Cells and Regenerative Medicine Institute, Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,14]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.1095\/biolreprod.116.146159"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1093\/toxsci\/kfr226"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1016\/j.biopha.2018.06.109"},{"doi-asserted-by":"publisher","key":"e_1_2_10_5_1","DOI":"10.1002\/jcp.27862"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.1371\/journal.pone.0198742"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.1016\/s0093-691x(02)01082-8"},{"doi-asserted-by":"publisher","key":"e_1_2_10_8_1","DOI":"10.1016\/j.theriogenology.2019.03.028"},{"doi-asserted-by":"publisher","key":"e_1_2_10_9_1","DOI":"10.3109\/07435800903204082"},{"doi-asserted-by":"publisher","key":"e_1_2_10_10_1","DOI":"10.1128\/cdli.7.2.197-199.2000"},{"doi-asserted-by":"publisher","key":"e_1_2_10_11_1","DOI":"10.1016\/s0015-0282(98)00416-6"},{"doi-asserted-by":"publisher","key":"e_1_2_10_12_1","DOI":"10.1590\/s0100-879x2004001100021"},{"doi-asserted-by":"publisher","key":"e_1_2_10_13_1","DOI":"10.1080\/09513590.2018.1431770"},{"doi-asserted-by":"publisher","key":"e_1_2_10_14_1","DOI":"10.1177\/1933719117698578"},{"doi-asserted-by":"publisher","key":"e_1_2_10_15_1","DOI":"10.1007\/s10815-018-1317-z"},{"doi-asserted-by":"publisher","key":"e_1_2_10_16_1","DOI":"10.1016\/j.fertnstert.2009.03.040"},{"doi-asserted-by":"publisher","key":"e_1_2_10_17_1","DOI":"10.1016\/j.lfs.2014.06.007"},{"doi-asserted-by":"publisher","key":"e_1_2_10_18_1","DOI":"10.1530\/rep.1.00735"},{"doi-asserted-by":"publisher","key":"e_1_2_10_19_1","DOI":"10.1016\/s0092-8674(00)80402-x"},{"doi-asserted-by":"publisher","key":"e_1_2_10_20_1","DOI":"10.3945\/an.110.000125"},{"doi-asserted-by":"publisher","key":"e_1_2_10_21_1","DOI":"10.1080\/14647273.2018.1466400"},{"doi-asserted-by":"publisher","key":"e_1_2_10_22_1","DOI":"10.1016\/s1472-6483(10)61978-5"},{"doi-asserted-by":"publisher","key":"e_1_2_10_23_1","DOI":"10.15171\/apb.2016.010"},{"doi-asserted-by":"publisher","key":"e_1_2_10_24_1","DOI":"10.1016\/j.theriogenology.2019.11.028"},{"doi-asserted-by":"publisher","key":"e_1_2_10_25_1","DOI":"10.1016\/j.ydbio.2005.08.023"},{"doi-asserted-by":"publisher","key":"e_1_2_10_26_1","DOI":"10.1080\/10408398.2016.1203286"},{"doi-asserted-by":"publisher","key":"e_1_2_10_27_1","DOI":"10.1084\/jem.62.5.665"},{"doi-asserted-by":"publisher","key":"e_1_2_10_28_1","DOI":"10.1016\/j.jsbmb.2015.07.023"},{"doi-asserted-by":"publisher","key":"e_1_2_10_29_1","DOI":"10.1093\/molehr\/gax032"},{"doi-asserted-by":"publisher","key":"e_1_2_10_30_1","DOI":"10.1007\/s10815-010-9523-3"},{"doi-asserted-by":"publisher","key":"e_1_2_10_31_1","DOI":"10.1007\/s10815-010-9523-3"},{"doi-asserted-by":"publisher","key":"e_1_2_10_32_1","DOI":"10.1095\/biolreprod.102.012096"},{"doi-asserted-by":"publisher","key":"e_1_2_10_33_1","DOI":"10.1016\/j.fertnstert.2013.01.129"},{"doi-asserted-by":"publisher","key":"e_1_2_10_34_1","DOI":"10.1093\/humrep\/deu248"},{"doi-asserted-by":"publisher","key":"e_1_2_10_35_1","DOI":"10.7150\/jca.15403"},{"doi-asserted-by":"publisher","key":"e_1_2_10_36_1","DOI":"10.1262\/jrd.09-199h"},{"doi-asserted-by":"publisher","key":"e_1_2_10_37_1","DOI":"10.3390\/ani9060374"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.29834","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.29834","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.29834","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.29834","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T02:51:16Z","timestamp":1692413476000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.29834"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,14]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1002\/jcp.29834"],"URL":"https:\/\/doi.org\/10.1002\/jcp.29834","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"type":"print","value":"0021-9541"},{"type":"electronic","value":"1097-4652"}],"subject":[],"published":{"date-parts":[[2020,6,14]]},"assertion":[{"value":"2020-02-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}