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An increase in the expression of two muscle\u2010specific E3 ligases, atrogin\u20101 and MuRF\u20101, and oxidative stress are involved in muscle atrophy. Patients with chronic liver diseases (CLD) develop muscle wasting. Several bile acids increase in plasma during cholestatic CLD, among them, cholic acid (CA) and deoxycholic acid (DCA). The receptor for bile acids, TGR5, is expressed in healthy skeletal muscles. TGR5 is involved in the regulation of muscle differentiation and metabolic changes. In this paper, we evaluated the participation of DCA and CA in the generation of an atrophic condition in myotubes and isolated fibers from the muscle extracted from wild\u2010type (WT) and TGR5\u2010deficient (TGR5\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) male mice. The results show that DCA and CA induce a decrease in diameter, and myosin heavy chain (MHC) protein levels, two typical atrophic features in C\n                    <jats:sub>2<\/jats:sub>\n                    C\n                    <jats:sub>12<\/jats:sub>\n                    myotubes. We also observed similar results when INT\u2010777 agonists activated the TGR5 receptor. To evaluate the participation of TGR5 in muscle atrophy induced by DCA and CA, we used a culture of muscle fiber isolated from WT and TGR5\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice. Our results show that DCA and CA decrease the fiber diameter and MHC protein levels, and there is an increase in atrogin\u20101, MuRF\u20101, and oxidative stress in WT fibers. The absence of TGR5 in fibers abolished all these effects induced by DCA and CA. Thus, we demonstrated that CS and deoxycholic acid induce skeletal muscle atrophy through TGR5 receptor.\n                  <\/jats:p>","DOI":"10.1002\/jcp.29839","type":"journal-article","created":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T13:20:11Z","timestamp":1591536011000},"page":"260-272","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["Cholic acid and deoxycholic acid induce skeletal muscle atrophy through a mechanism dependent on TGR5 receptor"],"prefix":"10.1002","volume":"236","author":[{"given":"Johanna","family":"Abrigo","sequence":"first","affiliation":[{"name":"Laboratory of Muscle Pathology, Fragility suand Aging, Department of Biological Sciences, Faculty of Life Sciences Universidad Andres Bello Universidad Andres Bello Santiago Chile"},{"name":"Millennium Institute on Immunology and Immunotherapy Santiago Chile"},{"name":"Center for the Development of Nanoscience and Nanotechnology (CEDENNA) Universidad de Santiago de Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Laboratory of Muscle Pathology, Fragility suand Aging, Department of Biological Sciences, Faculty of Life Sciences Universidad Andres Bello Universidad Andres Bello Santiago Chile"},{"name":"Millennium Institute on Immunology and Immunotherapy Santiago Chile"},{"name":"Center for the Development of Nanoscience and Nanotechnology (CEDENNA) Universidad de Santiago de Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Aguirre","sequence":"additional","affiliation":[{"name":"Laboratory of Muscle Pathology, Fragility suand Aging, Department of Biological Sciences, Faculty of Life Sciences Universidad Andres Bello Universidad Andres Bello Santiago Chile"},{"name":"Millennium Institute on Immunology and Immunotherapy Santiago 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Chile"},{"name":"Millennium Institute on Immunology and Immunotherapy Santiago Chile"},{"name":"Center for the Development of Nanoscience and Nanotechnology (CEDENNA) Universidad de Santiago de Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Paz","family":"Meza","sequence":"additional","affiliation":[{"name":"Laboratory of Muscle Pathology, Fragility suand Aging, Department of Biological Sciences, Faculty of Life Sciences Universidad Andres Bello Universidad Andres Bello Santiago Chile"},{"name":"Millennium Institute on Immunology and Immunotherapy Santiago Chile"},{"name":"Center for the Development of Nanoscience and Nanotechnology (CEDENNA) Universidad de Santiago de Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Simon","sequence":"additional","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy Santiago Chile"},{"name":"Millennium Nucleus of Ion 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