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Senescence of PDLSCs occurred during long\u2010term culture may compromise the therapeutic effects of PDLSCs. Medium supplements may be useful in antisenescence. However, the effects and mechanisms of vitamin C (Vc) treatment on PDLSCs during long\u2010term culture are still unclear. In this study, we identified that Vc\u2010treated PDLSCs cells maintained a slender morphology, higher growth rate and migration capacity, stemness, and osteogenic differentiation capability during a long\u2010term culture. Moreover, we also identified that Notch3 was significantly upregulated during the cell senescence, and Vc treatment alleviated the senescence of PDLSCs through inhibition of Notch3 during long\u2010term culture. In summary, Vc treatment suppressed PDLSCs senescence by reducing the expression of Notch3 and might be a simple and useful strategy to inhibit cellular senescence during the cell long\u2010term culture.<\/jats:p>","DOI":"10.1002\/jcp.29930","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T05:45:40Z","timestamp":1594705540000},"page":"1237-1251","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Vitamin C alleviates the senescence of periodontal ligament stem cells through inhibition of Notch3 during long\u2010term culture"],"prefix":"10.1002","volume":"236","author":[{"given":"Yan","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Disease, West China Hospital of Stomatology Sichuan University Chengdu China"},{"name":"National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology Sichuan University Chengdu 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