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The effect of biochanin A (BCA) has been examined in osteoporosis, but no study on the role of BCA in prosthetic loosening osteolysis has been conducted yet. In this study, we utilised enzyme\u2010linked immunosorbent assay, computed tomography imaging, and histological analysis. Results showed that BCA downregulated the secretion levels of tumor necrosis factor\u2010\u03b1, interleukin\u20101\u03b1 (IL\u20101\u03b1), and IL\u20101\u03b2 to suppress inflammatory responses. The secretion levels of receptor\u2010activated nuclear factor\u2010\u03baB ligand, CTX\u20101, and osteoclast\u2010associated receptor as well as Ti\u2010induced osteolysis were also reduced. BCA effectively inhibited osteoclastogenesis and suppressed hydroxyapatite resorption by downregulating OC\u2010related genes in vitro. Analysis of mechanisms indicated that BCA inhibited the signalling pathways of mitogen\u2010activated protein kinase (P38, extracellular signal\u2010regulated kinase, and c\u2010JUN N\u2010terminal kinase) and nuclear factor\u2010\u03baB (inhibitor \u03baB\u2010\u03b1 and P65), thereby downregulating the expression of nuclear factor of activated T cell 1 and c\u2010Fos. In conclusion, BCA may be an alternative choice for the prevention of prosthetic loosening caused by OCs.<\/jats:p>","DOI":"10.1002\/jcp.29948","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T18:31:26Z","timestamp":1598553086000},"page":"1432-1444","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Inhibitory effects of biochanin A on titanium particle\u2010induced osteoclast activation and inflammatory bone resorption via NF\u2010\u03baB and MAPK pathways"],"prefix":"10.1002","volume":"236","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3094-0883","authenticated-orcid":false,"given":"Shijie","family":"Liao","sequence":"first","affiliation":[{"name":"Department of Orthopaedics First Affiliated Hospital of Guangxi Medical University Nanning Guangxi China"},{"name":"Research Centre for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine Guangxi Medical 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