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It is essential to identify the medications that can effectively suppress the excessive differentiation and function of osteoclasts to prevent and reduce the osteolytic conditions. It has been reported that Carnosol (Car), isolated from rosemary and salvia, has anti\u2010inflammatory, antioxidative, and anticancer effects, but its activity on osteolysis has not been determined. In this study, we found that Car has a strong inhibitory effect on the receptor activator of nuclear factor\u2010\u03baB ligand (RANKL)\u2010induced osteoclast differentiation dose\u2010dependently without any observable cytotoxicity. Moreover, Car can inhibit the RANKL\u2010induced osteoclastogenesis and resorptive function via suppressing NFATc1, which is a result of affecting MAPK, NF\u2010\u03baB and Ca<jats:sup>2+<\/jats:sup> signaling pathways. Moreover, the particle\u2010induced osteolysis mouse model confirmed that Car could be effective for the treatment of bone loss in vivo. Taken together, by suppressing the formation and function of RANKL\u2010induced osteoclast, Car, may be a therapeutic supplementary in the prevention or the treatment of osteolysis.<\/jats:p>","DOI":"10.1002\/jcp.29978","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T23:58:07Z","timestamp":1595980687000},"page":"1950-1966","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Carnosol suppresses RANKL\u2010induced osteoclastogenesis and attenuates titanium particles\u2010induced osteolysis"],"prefix":"10.1002","volume":"236","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4897-1194","authenticated-orcid":false,"given":"Yongxian","family":"Li","sequence":"first","affiliation":[{"name":"The First Clinical Academy Guangzhou University of Chinese Medicine Guangzhou China"},{"name":"The First Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou 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