{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:57:10Z","timestamp":1787504230121,"version":"build-2736575974"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T00:00:00Z","timestamp":1597017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study aimed to investigate the mechanism by which MALAT1 regulates CRY2 expression and participates in trophoblast migration and invasion. Three patients with unexplained recurrent spontaneous abortion, four patients with missed abortion, and four women who underwent artificial miscarriages were enrolled in this study. Quantitative reverse\u2010transcription polymerase chain reaction and western blot analysis were used to detect RNA and protein expression, respectively. Trophoblast migration and invasion were detected by wound\u2010healing and transwell invasion assays. RNA pull\u2010down and Co\u2010IP assays were used to indicate the interaction between MALAT1 and FBXW7 or the interaction between FBXW7 and CRY2. The results showed significantly decreased MALAT1 expression in the villous specimens from the RSA patients relative to that in the villous specimens from the missed abortion patients and the normal villous specimens. MALAT1 promoted trophoblast cell migration and invasion by negatively regulating CRY2 protein expression. MALAT1 recruited FBXW7 to impair CRY2 protein stability. In conclusion, MALAT1 downregulation in trophoblasts might be related to miscarriage. MALAT1 may recruit the E3 ubiquitin ligase FBXW7 to induce CRY2 ubiquitin\u2010mediated degradation and participate in trophoblast migration and invasion.<\/jats:p>","DOI":"10.1002\/jcp.30003","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T06:37:12Z","timestamp":1597041432000},"page":"2169-2177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["MALAT1 recruited the E3 ubiquitin ligase FBXW7 to induce CRY2 ubiquitin\u2010mediated degradation and participated in trophoblast migration and invasion"],"prefix":"10.1002","volume":"236","author":[{"given":"Lianzhi","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Obstetrics Renmin Hospital of Wuhan University Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Renmin Hospital of Wuhan University Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuifang","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Renmin Hospital of Wuhan University Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Renmin Hospital of Wuhan University Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1727-1726","authenticated-orcid":false,"given":"Biheng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Renmin Hospital of Wuhan University Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00385"},{"issue":"10","key":"e_1_2_8_3_1","first-page":"12718","article-title":"Long non\u2010coding RNA MALAT\u20101 is downregulated in preeclampsia and regulates proliferation, apoptosis, migration and invasion of JEG\u20103 trophoblast cells","volume":"8","author":"Chen H.","year":"2015","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12576-016-0486-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-15-0030"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-019-0265-0"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.10.012"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/7090767"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2016.11.001"},{"key":"e_1_2_8_10_1","first-page":"79","article-title":"CRY2 suppresses trophoblast migration and invasion in recurrent spontaneous abortion","volume":"167","author":"Lianzhi W.","year":"2019","journal-title":"The Journal of Biochemistry"},{"issue":"21","key":"e_1_2_8_11_1","first-page":"7105","article-title":"LncRNA\u2010TCL6 promotes early abortion and inhibits placenta implantation via the EGFR pathway","volume":"22","author":"Liu L. 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