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ADSCs were isolated from adipose tissue that was harvested under sterile conditions from the inguen fold of porcines and cultured in vitro. Acellular cartilage extracellular matrix (ACECM) scaffolds of pigs were then constructed. Moreover, inflammatory cells, as well as cellular and humoral immune responses, were detected using hematoxylin and eosin staining staining, immunohistochemical staining, and western blot analysis. The results showed that the cartilage complex constructed by ADSCs and ACECM through tissue engineering successfully repaired the cartilage defect of the pig knee joint. The in vivo repair experiment showed no significant difference between chondrocytes, ADSCs, and induced ADSCs, indicating that ADSCs do not require in vitro induction and have the potential for chondrogenic differentiation in the environment around the knee joint. 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ADSCs may serve as viable seed cells for cartilage tissue engineering.<\/jats:p>","DOI":"10.1002\/jcp.30020","type":"journal-article","created":{"date-parts":[[2021,2,20]],"date-time":"2021-02-20T20:47:40Z","timestamp":1613854060000},"page":"4244-4257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Repair of articular cartilage defect using adipose\u2010derived stem cell\u2010loaded scaffold derived from native cartilage extracellular matrix"],"prefix":"10.1002","volume":"236","author":[{"given":"Liang","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei Anhui Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xifu","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei Anhui Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei Anhui Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei Anhui Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital  Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedics, The Chinese People's Liberation Army General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Sui","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedics, The Chinese People's Liberation Army General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedics, The Chinese People's Liberation Army General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5700-2128","authenticated-orcid":false,"given":"Quanyi","family":"Guo","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedics, The Chinese People's Liberation Army General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23782"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.09.070"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2019.1664224"},{"key":"e_1_2_10_5_1","first-page":"487","article-title":"Effect of growth factors on cartilage repair","volume":"46","author":"Coutts R. 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