{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:20:25Z","timestamp":1790950825899,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T00:00:00Z","timestamp":1599350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014717","name":"National Outstanding Youth Science Fund Project of National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672206"],"award-info":[{"award-number":["81672206"]}],"id":[{"id":"10.13039\/100014717","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ferroptosis is a necrotic form of regulated cell death that was associated with lipid peroxidation and free iron\u2010mediated Fenton reactions. It has been reported that iron deficiency had been implicated in the pathogenesis of intervertebral disc degeneration (IVDD) by activating apoptosis. However, the role of ferroptosis in the process of IVDD has not been illuminated. Here, we demonstrate the involvement of ferroptosis in IVDD pathogenesis. Our in vitro models show the changes in protein levels of ferroptosis marker and enhanced lipid peroxidation level during oxidative stress. Safranin O staining, hematoxylin\u2010eosin staining, and immunohistochemical were used to assess the IVDD after 8 weeks of surgical procedure in vivo. Treatment with ferrostatin\u20101, deferoxamine, and RSL3 demonstrate the role of ferroptosis in <jats:italic>tert<\/jats:italic>\u2010butyl hydroperoxide (TBHP)\u2010treated annulus fibrosus cells (AFCs) and nucleus pulposus cells (NPCs). Ferritinophagy, nuclear receptor coactivator 4 (NCOA4)\u2010mediated ferritin selective autophagy, is originated during the process of ferroptosis in response to TBHP treatment. Knockdown and overexpression NCOA4 further prove TBHP may induce ferroptosis of AFCs and NPCs in an autophagy\u2010dependent way. These findings support a role for oxidative stress\u2010induced ferroptosis in the pathogenesis of IVDD.<\/jats:p>","DOI":"10.1002\/jcp.30039","type":"journal-article","created":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T08:16:57Z","timestamp":1599380217000},"page":"2725-2739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":207,"title":["Involvement of oxidative stress\u2010induced annulus fibrosus cell and nucleus pulposus cell ferroptosis in intervertebral disc degeneration pathogenesis"],"prefix":"10.1002","volume":"236","author":[{"given":"Run\u2010Ze","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Clinic of Spine Center, Xinhua Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Ning","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinic of Spine Center, Xinhua Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huo\u2010Liang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Clinic of Spine Center, Xinhua Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Feng","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Clinic of Spine Center, Xinhua Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinic of Spine Center, Xinhua Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei\u2010Sheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Clinic of Spine Center, Xinhua Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6653-1881","authenticated-orcid":false,"given":"Sheng\u2010Dan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Clinic of Spine Center, Xinhua Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2017.02.005"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2018.06.010"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.07.028"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2014.11.021"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2017-212056"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-019-0408-1"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.22203\/ecm.v030a07"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3053"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2016.1152433"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3064"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.4161\/onci.20297"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2016.95"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.06.011"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e31817c64a9"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2017.11.009"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2016.1187366"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0006-291x(03)00734-4"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1017190"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000851"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2013.06.099"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci73942"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.05.074"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29571"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.90777"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3743"},{"issue":"1","key":"e_1_2_8_28_1","first-page":"47","article-title":"Degeneration of the intervertebral disc with new approaches for treating low back pain","volume":"59","author":"Le Maitre C. L.","year":"2015","journal-title":"Journal of Neurosurgical Sciences"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.10308"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13148"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1681\/asn.2015121376"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22320"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1397.090"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14191"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27444"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.09.021"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22204"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201900703R"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2014.06.006"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.07.050"},{"issue":"3","key":"e_1_2_8_41_1","first-page":"14","article-title":"World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects","volume":"81","author":"World Medical Association","year":"2014","journal-title":"Journal of the American College of Dentists"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2015.158"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2017.01.005"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.06.163"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13254"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12847"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102266"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.30039","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.30039","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.30039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T11:17:34Z","timestamp":1732792654000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.30039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,6]]},"references-count":46,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1002\/jcp.30039"],"URL":"https:\/\/doi.org\/10.1002\/jcp.30039","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,6]]},"assertion":[{"value":"2020-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}