{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:43:25Z","timestamp":1790621005129,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,9,22]],"date-time":"2020-09-22T00:00:00Z","timestamp":1600732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Glucagon\u2010like peptide\u20102 (GLP\u20102), an intestinotrophic hormone, has drawn considerable attention worldwide due to its potential to promote intestinal development. We investigated the effects and mechanisms of GLP\u20102 against lipopolysaccharide (LPS)\u2010induced intestinal inflammation and injury both in vitro and in vivo. Forty healthy piglets weaned at the age of 28 days with similar body weight (BW) were assigned to four in vivo treatments with ten piglets each: (i) nonchallenged control; (ii) LPS\u2010challenged control; (iii) LPS\u2009+\u2009low dose GLP\u20102; and (iv) LPS\u2009+\u2009high dose GLP\u20102. Piglets were subcutaneously injected with phosphate\u2010buffered saline supplemented with GLP\u20102 at doses of 0, 0, 2, and 10\u2009nmol\/kg\u2009BW per day for seven consecutive days. The piglets were challenged with an intraperitoneal injection with 100\u2009\u03bcg\/kg LPS on day 14 to induce intestinal damage. After that, the gene and protein expression levels of representative tight junction proteins and myosin light\u2010chain kinase (MLCK)\/phosphorylated myosin light chain (pMLC), as well as proinflammatory cytokine levels were determined using quantitative reverse transcription polymerase chain reaction, western blot, and enzyme\u2010linked immunosorbent assay methods. A high dose of GLP\u20102 pretreatment increased intestinal permeability by downregulating and redistributing tight junction proteins (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.05), for example, zona occluden\u20101 (ZO\u20101) and occludin. GLP\u20102 decreased the transcription of proinflammatory cytokines genes including interleukin\u20101\u03b2 (IL\u20101\u03b2), IL\u20106, IL\u20108, and tumor necrosis factor\u2010\u03b1 in small intestines (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.05). GLP\u20102 prevented the LPS\u2010induced increase in the expression of MLCK dose\u2010dependently and the increase in pMLC levels in the duodenum, jejunum, and ileum. To assess further the protective effect of GLP\u20102 on LPS\u2010induced intestinal barrier injury after weaning and its possible mechanism, an in vitro intestinal epithelial barrier model was established with IPEC\u2010J2 monolayers and treated with 100\u2009\u03bcg\/ml LPS with or without 1\u2009\u00d7\u200910\n                    <jats:sup>\u22128<\/jats:sup>\n                    \u2009mol\/L GLP\u20102 pretreatment. The in vitro analysis included control, LPS, and GLP\u20102\u2009+\u2009LPS treatments. GLP\u20102 treatment alleviated the destructive effect of LPS on barrier permeability by restoring the expression and ultrastructure of ZO\u20101 and occludin (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.05). In addition, GLP\u20102 reversed the LPS\u2010induced MLCK hyperexpression and pMLC hyperphosphorylation (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.05). Taken together, our findings revealed a mechanism by which GLP\u20102 alleviated LPS\u2010challenged intestinal barrier injury and inflammation in weaned piglets and IPEC\u2010J2 cells via the MLCK\/pMLC signaling pathway.\n                  <\/jats:p>","DOI":"10.1002\/jcp.30068","type":"journal-article","created":{"date-parts":[[2020,9,22]],"date-time":"2020-09-22T19:25:00Z","timestamp":1600802700000},"page":"3015-3032","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Glucagon\u2010like peptide 2 attenuates intestinal mucosal barrier injury through the MLCK\/pMLC signaling pathway in a piglet model"],"prefix":"10.1002","volume":"236","author":[{"given":"Yaqi","family":"Chang","sequence":"first","affiliation":[{"name":"Institute of Animal Nutrition Sichuan Agricultural University Chengdu Sichuan China"},{"name":"Key Laboratory of Animal Disease\u2010resistant Nutrition  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuhong","family":"Deng","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Sichuan Agricultural University Chengdu Sichuan China"},{"name":"Key Laboratory of Animal Disease\u2010resistant Nutrition  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Sichuan Agricultural University Chengdu Sichuan China"},{"name":"Key Laboratory of Animal Disease\u2010resistant Nutrition  Chengdu China"},{"name":"Meishan Vocational Technical College Meishan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Sichuan Agricultural University Chengdu Sichuan China"},{"name":"Key Laboratory of Animal Disease\u2010resistant Nutrition  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayong","family":"Tang","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Sichuan Agricultural University Chengdu Sichuan China"},{"name":"Key Laboratory of Animal Disease\u2010resistant Nutrition  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Sichuan Agricultural University Chengdu Sichuan China"},{"name":"Key Laboratory of Animal Disease\u2010resistant Nutrition  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangmang","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Sichuan Agricultural University Chengdu Sichuan China"},{"name":"Key Laboratory of Animal Disease\u2010resistant Nutrition  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Tian","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Sichuan Agricultural University Chengdu Sichuan China"},{"name":"Key Laboratory of Animal Disease\u2010resistant Nutrition  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Cai","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Sichuan Agricultural University Chengdu Sichuan China"},{"name":"Key Laboratory of Animal Disease\u2010resistant Nutrition  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1102-0683","authenticated-orcid":false,"given":"Gang","family":"Jia","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Sichuan Agricultural University Chengdu Sichuan China"},{"name":"Key Laboratory of Animal Disease\u2010resistant Nutrition  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2000.6861"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2015.12.016"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.7.4641"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1999.277.5.E937"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c170055"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1119"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.085373"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2008.165886"},{"issue":"8","key":"e_1_2_11_10_1","first-page":"760","article-title":"Effects of GLP\u20102 on the intestinal epithelium tight junction in rats of obstructive jaundice","volume":"17","author":"Chen Z. Y.","year":"2008","journal-title":"Chinese Journal of Current Advances in General Surgery"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-016-0087-7"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.2002.6415"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0797-673"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.276.1.G79"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-23-j0004.2000"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795-013-0055-7"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.123.6.1777"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-011-9362-9"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1402598"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-1228"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200303000-00006"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-011-1191-9"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5796"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/07435800.2018.1460604"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/0148607115597644"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00167-0"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2017.10.007"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17020190"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114508911612"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110881"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.109.161497"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2015.07.025"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2015.40"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.6.1781"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2013.12.006"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.13118"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04054.x"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12603-018-1022-8"},{"issue":"1","key":"e_1_2_11_40_1","first-page":"58","article-title":"Changes of plasma D(\u2010)\u2010lactate, diamine oxidase and endotoxin in patients with liver cirrhosis","volume":"3","author":"Ruan P.","year":"2004","journal-title":"Hepatobiliary & Pancreatic Diseases International"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-13-0184"},{"issue":"10","key":"e_1_2_11_42_1","first-page":"2095","article-title":"Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure","volume":"119","author":"Shen L.","year":"2006","journal-title":"Journal of Microscopical Science"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00530.2006"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.10.081"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.4.C1378"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2014-8919"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114510005660"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.262.5.C1119"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1017\/S000711451800003X"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114520000082"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00177.2003"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00318.2005"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.15.0415"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.30068","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.30068","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.30068","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T06:20:21Z","timestamp":1732774821000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.30068"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,22]]},"references-count":52,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1002\/jcp.30068"],"URL":"https:\/\/doi.org\/10.1002\/jcp.30068","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,22]]},"assertion":[{"value":"2020-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}