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Whether deficient muscle repair mechanisms and regeneration exist in the vastus lateralis (VL) of sarcopenic COPD remains debatable. In the VL of control subjects and severe COPD patients with\/without sarcopenia, satellite cells (SCs) were identified (immunofluorescence, specific antibodies, anti\u2010Pax\u20107, and anti\u2010Myf\u20105): activated (Pax\u20107+\/Myf\u20105+), quiescent\/regenerative potential (Pax\u20107+\/Myf\u20105\u2010), and total SCs, nuclear activation (terminal deoxynucleotidyl transferase\u2010mediated dUTP nick\u2010end labeling [TUNEL]), and muscle fiber type (morphometry and slow\u2010 and fast\u2010twitch, and hybrid fibers), muscle damage (hematoxylin\u2010eosin staining), muscle regeneration markers (Pax\u20107, Myf\u20105, myogenin, and MyoD), and myostatin levels were identified. Compared to controls, in VL of sarcopenic COPD patients, myostatin content, activated SCs, hybrid fiber proportions, TUNEL\u2010positive cells, internal nuclei, and muscle damage significantly increased, while quadriceps muscle strength, numbers of Pax\u20107+\/Myf\u20105\u2010 and slow\u2010 and fast\u2010twitch, and hybrid myofiber areas decreased. In the VL of sarcopenic and nonsarcopenic patients, TUNEL\u2010positive cells were greater, whereas muscle regeneration marker expression was lower than in controls. In VL of severe COPD patients regardless of the sarcopenia level, the muscle regeneration process is triggered as identified by SC activation and increased internal nuclei. Nonetheless, a lower regenerative potential along with significant alterations in muscle phenotype and damage, and increased myostatin were prominently seen in sarcopenic COPD.<\/jats:p>","DOI":"10.1002\/jcp.30073","type":"journal-article","created":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T04:21:14Z","timestamp":1601353274000},"page":"3083-3098","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Deficient muscle regeneration potential in sarcopenic COPD patients: Role of satellite cells"],"prefix":"10.1002","volume":"236","author":[{"given":"Antonio","family":"Sancho\u2010Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Pulmonology Department, Muscle Wasting and Cachexia in Chronic Respiratory Diseases and Lung Cancer Research Group IMIM\u2010Hospital del Mar, Parc de Salut Mar  Barcelona Spain"},{"name":"Health and Experimental Sciences Department (CEXS), Universitat Pompeu Fabra (UPF) Barcelona Biomedical Research Park (PRBB)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Guitart","sequence":"additional","affiliation":[{"name":"Pulmonology Department, Muscle Wasting and Cachexia in Chronic Respiratory Diseases and Lung Cancer Research Group IMIM\u2010Hospital del Mar, Parc de Salut Mar  Barcelona Spain"},{"name":"Health and Experimental Sciences Department (CEXS), Universitat Pompeu Fabra (UPF) Barcelona Biomedical Research Park (PRBB)  Barcelona Spain"},{"name":"Centro de Investigaci\u00f3n en Red de Enfermedades Respiratorias (CIBERES) Instituto de Salud Carlos III (ISCIII)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego A.","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Pulmonology Department, Muscle Wasting and Cachexia in Chronic Respiratory Diseases and Lung Cancer Research Group IMIM\u2010Hospital del Mar, Parc de Salut Mar  Barcelona 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