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MSC is an important part of the \u201ccolon cancer stem cell niche\u201d, but little research has been done on the tropism of human MSCs toward colon cancer stem cells (CCSCs). In this study, we first compared the effects of three tissue\u2010derived MSCs (bone marrow, adipose tissue, and placenta) in vivo on colon tumor xenograft growth. Then, we analyzed the tropism of bone marrow\u2010derived MSCs (BMSCs) toward normal intestinal epithelial cells (NCM460), parental colon cancer cells, CD133<jats:sup>\u2212<\/jats:sup>\/CD44<jats:sup>\u2212,<\/jats:sup> and CD133<jats:sup>+<\/jats:sup>\/CD44<jats:sup>+<\/jats:sup> colon cancer cells in vitro. Microarray analysis and in vitro experiments explored the mechanism of mediating the homing of BMSCs toward CCSCs. Compared with the parental and CD133<jats:sup>\u2212<\/jats:sup>\/CD44<jats:sup>\u2212<\/jats:sup> colon cancer cells, CD133<jats:sup>+<\/jats:sup>\/CD44<jats:sup>+<\/jats:sup> cells have a stronger ability to recruit BMSCs. In addition, BMSCs were significantly transformed into cancer\u2010associated fibroblasts after being recruited by CCSCs. After coculture of BMSCs and CCSCs, the expression of interleukin (IL)\u20106, IL\u20108, IL\u201032, and CCL20 was significantly increased. Compared with parental strains, CD133<jats:sup>\u2212<\/jats:sup>\/CD44<jats:sup>\u2212<\/jats:sup> cells, and NCM460, BMSC secreted significantly more IL\u20108 after coculture with CD133<jats:sup>+<\/jats:sup>\/CD44<jats:sup>+<\/jats:sup> cells. Low concentration of IL\u20108 peptide inhibitors (100\u2009ng\/ml) and CXC receptor 2 (CXCR2) inhibitors have little effect on the migration of BMSCs, but can effectively weaken CCSC stemness and promote dormant CSCs in the coculture system to re\u2010enter into the cell cycle. The endogenous IL\u20108 knockout in BMSCs or BMSCs loaded with IL\u20108 and\/or CXCR2 inhibitors will make the therapy of BMSC targeting CCSCs function at its best.<\/jats:p>","DOI":"10.1002\/jcp.30080","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T07:08:48Z","timestamp":1603177728000},"page":"3114-3128","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["IL\u20108\/CXCR2 mediates tropism of human bone marrow\u2010derived mesenchymal stem cells toward CD133<sup>+<\/sup>\/CD44<sup>+<\/sup> Colon cancer stem cells"],"prefix":"10.1002","volume":"236","author":[{"given":"Xiaoying","family":"Ma","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering &amp; Shanghai Key Laboratory of New Drug Design, School of Pharmacy East China University of Science and Technology  Shanghai 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