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Recent studies have demonstrated that TQ contributes to the suppression of liver fibrosis. Abnormal activated epithelial\u2010mesenchymal transition (EMT) promotes the activation of hepatic stellate cells (HSCs). However, whether the antifibrotic effects of TQ occur\u00a0through inhibiting EMT is largely unknown. In this study, it was found that TQ ameliorated liver fibrosis and collagen accumulation in carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) mice. In vitro, TQ inhibited HSC activation including reduced proliferation, \u03b1\u2010smooth muscle actin, and collagen. In addition, TQ markedly suppressed the EMT process, with enhanced E\u2010cadherin and reduced desmin. Notably, snail family transcriptional repressor 1 (Snai1), the EMT master transcription factor, was obviously inhibited by TQ in vivo and in vitro. Further studies demonstrated that Snai1 was a target of microRNA\u201030a (miR\u201030a), which was upregulated by TQ. Interestingly, the effects of TQ on HSC activation and EMT were almost inhibited by miR\u201030a inhibitor. Collectively, we demonstrate that TQ inhibits HSC activation, at least in part, via regulation of miR\u201030a and Snai1. TQ upregulates miR\u201030a expression, resulting in a reduced Snai1 level as well as EMT process inactivation, which contributes to the inhibition of HSC activation. TQ may be a potential therapeutic agent for liver fibrosis.<\/jats:p>","DOI":"10.1002\/jcp.30097","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T06:28:49Z","timestamp":1602224929000},"page":"3629-3640","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Thymoquinone alleviates liver fibrosis via miR\u201030a\u2010mediated epithelial\u2010mesenchymal transition"],"prefix":"10.1002","volume":"236","author":[{"given":"Wujun","family":"Geng","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunxue","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Diagnosis and Treatment of Severe Hepato\u2010Pancreatic Diseases of Zhejiang Province The First Affiliated Hospital of Wenzhou Medical University 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