{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T06:40:02Z","timestamp":1778568002016,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T00:00:00Z","timestamp":1605225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81860283"],"award-info":[{"award-number":["81860283"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671486 and 81460226; Funds of Health and Family Planning Commission of Jiangxi Province:20195050"],"award-info":[{"award-number":["81671486 and 81460226; Funds of Health and Family Planning Commission of Jiangxi Province:20195050"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recently, studies have shown that neuropathy target esterase (NTE) is essential to placental and normal blood vessel development. However, whether it is involved in abnormal placenta angiogenesis of pre\u2010eclampsia remains unknown. Thus, our aim was to observe the expression of NTE in pre\u2010eclamptic placentas and its effects and mechanism of NTE on the migration and the tube formation of human umbilical vein endothelial cells (HUVECs). Immunohistochemical staining showed that the NTE protein was intensely located in blood vessels of the normal pregnant placenta. However, western blot revealed that the expression level of NTE protein was significantly reduced in pre\u2010eclamptic placenta. The results indicated that overexpression of NTE significantly promoted the migration and the tube formation of HUVECs compared with those of the control and scramble short hairpin RNA (shRNA) group. Conversely, NTE shRNA obviously inhibited the migration and the tube formation of HUVECs. Additionally, chromatography assay evidenced that NTE overexpression significantly reduced the level of phosphatidylcholine (PC) of HUVECs, but NTE shRNA obviously increased the level of PC of HUVECs. Furthermore, exogenous PC and lysophosphatidylcholine (LPC) significantly inhibited the tube formation of HUVECs in a dose\u2010dependent manner. Collectively, our results suggest that reduced NTE in placenta may contribute to abnormal placenta angiogenesis of pre\u2010eclampsia via the dysregulation of PC and LPC metabolism.<\/jats:p>","DOI":"10.1002\/jcp.30160","type":"journal-article","created":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T08:26:49Z","timestamp":1605256009000},"page":"4435-4444","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Reduced neuropathy target esterase in pre\u2010eclampsia suppresses tube formation of HUVECs via dysregulation of phospholipid metabolism"],"prefix":"10.1002","volume":"236","author":[{"given":"Mo","family":"Li","sequence":"first","affiliation":[{"name":"Department of Physiology,\u00a0Basic Medical College Nanchang University  Nanchang Jiangxi People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Physiology,\u00a0Basic Medical College Nanchang University  Nanchang Jiangxi People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology,\u00a0Basic Medical College Nanchang University  Nanchang Jiangxi People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Physiology,\u00a0Basic Medical College Nanchang University  Nanchang Jiangxi People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siying","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Physiology,\u00a0Basic Medical College Nanchang University  Nanchang Jiangxi People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Physiology,\u00a0Basic Medical College Nanchang University  Nanchang Jiangxi People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Ling","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Jiangxi Provincial People's Hospital Affiliated Nanchang University  Nanchang Jiangxi People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuezhen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology, Medical Experimental Teaching Center Nanchang University  Nanchang Jiangxi People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4711-5449","authenticated-orcid":false,"given":"Haibin","family":"Kuang","sequence":"additional","affiliation":[{"name":"Department of Physiology,\u00a0Basic Medical College Nanchang University  Nanchang Jiangxi People's Republic of China"},{"name":"Department of Reproductive Medicine, Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology, Medical Experimental Teaching Center Nanchang University  Nanchang Jiangxi People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27189"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom9120848"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.12.007"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2015.03.013"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.035600"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2014.11.009"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4274(83)90162-5"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2017.10.021"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2008.07.061"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms14023487"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1140711"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4196\/kjpp.2009.13.1.27"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3320001"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/009841096161276"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.4.1667-1679.2004"},{"issue":"3","key":"e_1_2_9_17_1","first-page":"241","article-title":"Development of trophoblast and placenta of the mouse. 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