{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T20:40:34Z","timestamp":1772052034649,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T00:00:00Z","timestamp":1607644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The rapid spread of coronavirus disease 2019 (COVID\u201019), a disease caused by severe acute respiratory syndrome coronavirus 2, poses a huge demand for immediate diagnosis. Real\u2010time reverse transcriptase\u2010polymerase chain reaction (rRT\u2010PCR) of nasopharyngeal (NP) and oropharyngeal (OP) swabs have been used to confirm the clinical diagnosis. To avoid the risk of viral\u2010exposure of laboratory workers, thermal inactivation is currently recommended but has unknown effects on the accuracy of the rRT\u2010PCR results. Thirty\u2010six NP\/OP specimens were collected from COVID\u201019 patients and subjected to thermal inactivation (60\u00b0C for 30\u2009min) or the RNA extraction processes to activate the form. Here, our data showed that the concentration of extracted\u2010RNA increases upon thermal inactivation compared to the active form (<jats:italic>p<\/jats:italic>\u2009=\u2009.028). \u00a0Significantly higher levels of RNA copy number were obtained in inactivated compared to the active samples for both N and ORF1ab genes (<jats:italic>p<\/jats:italic>\u2009=\u2009.009, <jats:italic>p<\/jats:italic>\u2009=\u2009.032, respectively). Thermal inactivation elevated concentration and copy number of extracted\u2010RNA, possibly through viral\u2010capsid degradation and\/or nucleoprotein denaturation.<\/jats:p>","DOI":"10.1002\/jcp.30206","type":"journal-article","created":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T14:20:00Z","timestamp":1607696400000},"page":"4966-4972","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Thermal inactivation of COVID\u201019 specimens improves RNA quality and quantity"],"prefix":"10.1002","volume":"236","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3869-598X","authenticated-orcid":false,"given":"Maral","family":"Hemati","sequence":"first","affiliation":[{"name":"Cancer Research Center Semnan University of Medical Sciences Semnan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohsen","family":"Soosanabadi","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics Semnan University of Medical Sciences Semnan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahereh","family":"Ghorashi","sequence":"additional","affiliation":[{"name":"COVID\u201019 Diagnostic Lab Semnan University of Medical Sciences Semnan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadi","family":"Ghaffari","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Virology Semnan University of Medical Sciences Semnan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azadeh","family":"Vahedi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Virology Semnan University of Medical Sciences Semnan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaheh","family":"Sabbaghian","sequence":"additional","affiliation":[{"name":"Cancer Research Center Semnan University of Medical Sciences Semnan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Rasouli Nejad","sequence":"additional","affiliation":[{"name":"Cancer Research Center Semnan University of Medical Sciences Semnan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir","family":"Salati","sequence":"additional","affiliation":[{"name":"Nervous System Stem Cells Research Center Semnan University of Medical Sciences Semnan Iran"},{"name":"Department of Tissue Engineering and Applied Cell Sciences Semnan University of Medical Sciences Semnan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Navid","family":"Danaei","sequence":"additional","affiliation":[{"name":"Department of Pediatric Semnan University of Medical Sciences Semnan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parviz","family":"Kokhaei","sequence":"additional","affiliation":[{"name":"Cancer Research Center Semnan University of Medical Sciences Semnan Iran"},{"name":"Department of Oncology\u2010Pathology, BioClinicum Karolinska University Hospital Solna and Karolinska Institute Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,11]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Cascella M. 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