{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T16:52:36Z","timestamp":1779123156040,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,1,16]],"date-time":"2021-01-16T00:00:00Z","timestamp":1610755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteoarthritis (OA) is the most common joint disease. The surface of joint cartilage is a defensive and first affected structure of articular cartilage (AC) during the pathogenesis of OA. Alk5 signaling is critical for maintaining AC homeostasis, however, the role and underlying mechanism for the involvement of Alk5 signaling in the phenotypes of articular cartilage stem cells (ACSCs) at the surface of AC is still unclear. The role of <jats:italic>Alk5<\/jats:italic> in OA development was explored using an ACSCs\u2010specific <jats:italic>Alk5<\/jats:italic>\u2010deficient (cKO) mouse model. Alterations in cartilage structure were evaluated histologically. Senescence was detected by SA\u2010\u03b2\u2010gal, while reactive oxygen species (ROS), MitoTracker, and LysoTracker staining were used to detect changes related to senescence. In addition, mice were injected intra\u2010articularly with ganciclovir to limit\u00a0the detrimental roles of senescent cells (SnCs). <jats:italic>Alk5<\/jats:italic> cKO mice showed a decreased number of the slow\u2010cell cycle cells and less lubricant secretion at the surface accompanied with drastically accelerated cartilage degeneration under ageing and surgically induced OA conditions. Further studies showed that <jats:italic>Alk5<\/jats:italic> deficient ACSCs exhibited senescence\u2010like manifestations including decreased proliferation and differentiation, more SA\u2010\u03b2\u2010gal\u2010positive cells and ROS production, as well as significantly swollen mitochondria and lysosome breakdown. We further found that local limitation of the detrimental roles of SnCs can attenuate the development of posttraumatic OA. Taken together, our findings suggest that Alk5 signaling acts as an important regulator of the SnCs in the superficial layer during AC maintenance and OA initiation.<\/jats:p>","DOI":"10.1002\/jcp.30231","type":"journal-article","created":{"date-parts":[[2021,1,16]],"date-time":"2021-01-16T09:42:18Z","timestamp":1610790138000},"page":"5278-5292","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["TGF\u2010\u03b2\/Alk5 signaling prevents osteoarthritis initiation via regulating the senescence of articular cartilage stem cells"],"prefix":"10.1002","volume":"236","author":[{"given":"Qiaoyan","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Wound Repair and Rehabilitation Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Trauma Center, Research Institute of Surgery, Daping Hospital Army Medical University  Chongqing 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