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However, the mechanisms underlying tissue regeneration using patient\u2010derived dental pulp stem cells (DPSCs) are unclear. In this study, we investigated the levels of mRNA and protein expression related to cellular differentiation of Crouzon syndrome patient\u2010derived DPSCs (CS\u2010DPSCs) with a Gly338Arg fibroblast growth factor receptor 2 mutation. Multipotency\u2010related gene expression levels were equivalent in both healthy donor DPSCs and CS\u2010DPSCs. CS\u2010DPSCs showed higher <jats:italic>osteocalcin<\/jats:italic> (<jats:italic>OCN<\/jats:italic>) expression than healthy donor DPSCs. CS\u2010DPSCs showed a lower increase in the rate of <jats:italic>OCN<\/jats:italic> expression among phorbol 12\u2010myristate 13\u2010acetate (PMA)\u2010treated cells than healthy donor DPSCs compared with untreated control cells. CS\u2010DPSCs showed a lower phosphorylation rate of p38 and p44\/42 in PMA\u2010treated cells than healthy donor DPSCs compared with untreated control cells. These results demonstrate that CS\u2010DPSCs have higher <jats:italic>OCN<\/jats:italic> expression and lower PMA stimulation\u2010responsiveness than healthy donor DPSCs.<\/jats:p>","DOI":"10.1002\/jcp.30241","type":"journal-article","created":{"date-parts":[[2021,1,2]],"date-time":"2021-01-02T04:34:30Z","timestamp":1609562070000},"page":"5317-5324","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of dental pulp stem cells isolated from a patient diagnosed with Crouzon syndrome"],"prefix":"10.1002","volume":"236","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7411-2556","authenticated-orcid":false,"given":"Daisuke","family":"Torii","sequence":"first","affiliation":[{"name":"Department of Pharmacology The Nippon Dental University School of Life Dentistry Tokyo 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