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Here, we investigated the megakaryo\/thrombopoiesis process, including the involvement of the c\u2010Mpl\/IEX\u20101 pathway, after HCMV infection, identified receptors mediating the interaction between megakaryocytes (MKs) and HCMV, and explored novel therapeutic targets. Our data shows that HCMV directly infects megakaryocytes in patients with HCMV DNAemia and influences megakaryopoiesis via the c\u2010Mpl\/IEX\u20101 pathway throughout megakaryocyte maturation, apoptosis, and platelet generation in vivo and in vitro. After treatment with inhibitors of PDGFR\u03b1 and \u03b1v\u03b23, the HCMV infection rate in MKs was significantly reduced, suggesting that IMC\u20103G3 and anti\u2010\u03b1v\u03b23 are potential therapeutic alternatives for viral infection. In summary, our study proposes a possible mechanism and potential treatments for thrombocytopenia caused by HCMV infection and other viral diseases associated with abnormal hemostasis.<\/jats:p>","DOI":"10.1002\/jcp.30335","type":"journal-article","created":{"date-parts":[[2021,2,21]],"date-time":"2021-02-21T17:36:04Z","timestamp":1613928964000},"page":"6726-6741","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["HCMV modulates c\u2010Mpl\/IEX\u20101 pathway\u2010mediated megakaryo\/thrombopoiesis via PDGFR\u03b1 and \u03b1v\u03b23 receptors after allo\u2010HSCT"],"prefix":"10.1002","volume":"236","author":[{"given":"Fei\u2010Er","family":"Feng","sequence":"first","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology  Beijing China"},{"name":"National Clinical Research Center for Hematologic Disease  Beijing China"},{"name":"Beijing Key Laboratory of Hematopoietic Stem 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