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Here, we found urine glycine in patients with kidney calcium oxalate (CaOx) stone was significantly lower than that in healthy people via <jats:sup>1<\/jats:sup>H NMR spectra detection, and investigated the role and underlying mechanism of glycine in the regulation of CaOx stone formation. Our results showed that glycine could significantly attenuate ethylene glycol\u2010induced CaOx crystal depositions in rat kidney via decreasing urine oxalate and increasing urine citrate. Mechanism studies revealed that glycine could decrease urine oxalate through downregulating Slc26a6 expression, whereas increase urine citrate via inhibiting Nadc1 expression. Moreover, glycine decreased the protein expression of both Slc26a6 and Nadc1 via increasing the expression of miRNA\u2010411\u20103p, which directly bound to the 3\u2032\u2010untranslated regions of Slc26a6 and Nadc1 messenger RNAs, in vitro and in vivo. Together, our results revealed a novel role of glycine in the regulation of kidney CaOx crystal formation and provided a potential target for the treatment of kidney CaOx stone.<\/jats:p>","DOI":"10.1002\/jcp.30370","type":"journal-article","created":{"date-parts":[[2021,3,27]],"date-time":"2021-03-27T18:14:36Z","timestamp":1616868876000},"page":"6824-6835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Glycine suppresses kidney calcium oxalate crystal depositions via regulating urinary excretions of oxalate and citrate"],"prefix":"10.1002","volume":"236","author":[{"given":"Yu","family":"Lan","sequence":"first","affiliation":[{"name":"Department of Urology and Guangdong Key Laboratory of Urology The First Affiliated Hospital of Guangzhou Medical University  Guangzhou Guangdong China"},{"name":"Center for Reproductive Medicine, Key Laboratory for Major Obstetric Diseases of Guangdong Province Third Affiliated 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