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In this study, we hypothesised that CPCs derived from OA cartilage may be characterised by a distinct channelome. First, a global transcriptomic analysis using Affymetrix microarrays was performed. We studied the profiles of those ion channels and transporter families that may be relevant to chondroprogenitor cell physiology. Following validation of the microarray data with quantitative reverse transcription\u2010polymerase chain reaction, we examined the role of calcium\u2010dependent potassium channels in CPCs and observed functional large\u2010conductance calcium\u2010activated potassium (BK) channels involved in the maintenance of the chondroprogenitor phenotype. In line with our very recent results, we found that the KCNMA1 gene was upregulated in CPCs and observed currents that could be attributed to the BK channel. The BK channel inhibitor paxilline significantly inhibited proliferation, increased the expression of the osteogenic transcription factor RUNX2, enhanced the migration parameters, and completely abolished spontaneous Ca\n                    <jats:sup>2+<\/jats:sup>\n                    events in CPCs. Through characterisation of their channelome we demonstrate that CPCs are a distinct cell population but are highly similar to MSCs in many respects. This study adds key mechanistic data to the in\u2010depth characterisation of CPCs and their phenotype in the context of cartilage regeneration.\n                  <\/jats:p>","DOI":"10.1002\/jcp.30413","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T02:12:55Z","timestamp":1621390375000},"page":"7421-7439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Transcriptome\u2010based screening of ion channels and transporters in a migratory chondroprogenitor cell line isolated from late\u2010stage osteoarthritic cartilage"],"prefix":"10.1002","volume":"236","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9678-7420","authenticated-orcid":false,"given":"Csaba","family":"Matta","sequence":"first","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine University of Debrecen Debrecen Hungary"},{"name":"Department of Veterinary Preclinical 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