{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T01:57:47Z","timestamp":1772675867529,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T00:00:00Z","timestamp":1626739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tissue engineering aims to develop innovative approaches to repair tissue defects. The use of adipose\u2010derived stem cells (ASCs) in tissue regeneration was extensively investigated for osteochondrogenesis. Among the ASC population, ASCs expressing the CD146 were demonstrated to be multipotent and considered as perivascular stem cells, although the functional role of CD146 expression in these cells remains unclear. Herein, we investigated the influence of CD146 expression on osteochondrogenic differentiation of ASCs. Our results showed that, in two\u2010dimensional culture systems, sorted CD146<jats:sup>+<\/jats:sup> ASCs proliferated less and displayed higher adipogenic and chondrogenic potential than CD146<jats:sup>\u2212<\/jats:sup> ASCs. The latter demonstrated a higher osteogenic capacity. Besides this, CD146<jats:sup>+<\/jats:sup> ASCs in three\u2010dimensional Matrigel\/endothelial growth medium (EGM) cultures showed the highest angiogenic capability. When cultured in three\u2010dimensional collagen scaffolds, CD146<jats:sup>+<\/jats:sup> ASCs showed a spontaneous chondrogenic differentiation, further enhanced by the EGM medium's addition. Finally, CD146<jats:sup>\u2212<\/jats:sup> ASCs seeded on hexafluoroisopropanol silk scaffolds displayed a greater spontaneous osteogenetic capacity. Altogether, these findings demonstrated a functional and relevant influence of CD146 expression in ASC properties and osteochondrogenic commitment. Exploiting the combination of specific differentiation properties of ASC subpopulations and appropriate culture systems could represent a promising strategy to improve the efficacy of new regenerative therapies.<\/jats:p>","DOI":"10.1002\/jcp.30506","type":"journal-article","created":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T13:00:10Z","timestamp":1626786010000},"page":"589-602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["CD146 expression regulates osteochondrogenic differentiation of human adipose\u2010derived stem cells"],"prefix":"10.1002","volume":"237","author":[{"given":"Maria Giovanna","family":"Scioli","sequence":"first","affiliation":[{"name":"Anatomic Pathology, Department of Biomedicine and Prevention University of Rome Tor Vergata  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Storti","sequence":"additional","affiliation":[{"name":"Plastic 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