{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:30:27Z","timestamp":1792182627386,"version":"4.4.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T00:00:00Z","timestamp":1628640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hainan Province","doi-asserted-by":"publisher","award":["820MS169"],"award-info":[{"award-number":["820MS169"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81873993"],"award-info":[{"award-number":["81873993"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Merlin is known as a tumor suppressor, while its role in osteomyelitis remains unclear. This study aimed to investigate the role of Merlin in <jats:italic>Staphylococcus aureus<\/jats:italic>\u2010induced osteomyelitis and its underlying mechanisms. <jats:italic>S. aureus<\/jats:italic>\u2010induced osteomyelitis mouse model was established in Merlin<jats:sup>fl\/fl<\/jats:sup>Lyz2<jats:sup>cre\/+<\/jats:sup> and Merlin<jats:sup>fl\/fl<\/jats:sup>Lyz2<jats:sup>+\/+<\/jats:sup> mice. Bone marrow\u2013derived macrophages (BMDMs) were isolated and stimulated by lipopolysaccharide (LPS). Bioassays, including quantitative reverse transcription polymerase chain reaction (qRT\u2010PCR), Western blot analysis, and enzyme\u2010linked immunosorbent assays, were conducted to determine the levels of target genes or proteins. Immunoprecipitation was applied to determine the interactions between proteins. DCAF1<jats:sup>fl\/fl<\/jats:sup> mice were further crossed with Lyz2\u2010Cre mice to establish myeloid cell conditional knockout mice (DCAF1<jats:sup>fl\/fl<\/jats:sup>Lyz2<jats:sup>cre\/+<\/jats:sup>). It was found that the level of Merlin was elevated in patients with osteomyelitis and <jats:italic>S. aureus<\/jats:italic>\u2010infected BMDMs. Merlin deficiency in macrophages suppressed the production of inflammatory cytokines and ameliorated the symptoms of osteomyelitis induced by <jats:italic>S. aureus<\/jats:italic>. Merlin deficiency in macrophages also suppressed the production of proinflammatory cytokines in BMDMs induced by LPS. The inhibitory effects of Merlin deficiency on the inflammatory response were associated with DDB1\u2010Cul4\u2010associated factor 1 (DCAF1). In summary, Merlin deficiency ameliorates <jats:italic>S. aureus<\/jats:italic>\u2010induced osteomyelitis through the regulation of DCAF1.<\/jats:p>","DOI":"10.1002\/jcp.30550","type":"journal-article","created":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T10:06:15Z","timestamp":1628676375000},"page":"815-823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Merlin functions as a critical regulator in <i>Staphylococcus aureus<\/i>\u2010induced osteomyelitis"],"prefix":"10.1002","volume":"237","author":[{"given":"Zubin","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanliang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Haikou Orthopedic and Diabetes Hospital of 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