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To understand the molecular mechanisms underlying this delay, microRNA (miRNA) profiling was performed on split skin biopsies collected on Day 1 (basal level) and Day 14 (wound re\u2010epithelialization) from nonlesional (NL) and lesional (L) skin of five subjects with stable\u00a0nonsegmental vitiligo and 129 miRNAs were found to be differentially regulated between the NL and L healed epidermis. miR\u201021\u20105p, expressed at comparable levels on NL and L Day 1 samples, demonstrated significant upregulation during re\u2010epithelialization. However, the extent of its upregulation was relatively lower in L (10 times compared to Day 1) as compared to NL skin (17 times compared to Day 1). The overexpression of miR\u201021 in keratinocytes led to a significant increase in the expression of proliferation markers (Ki67 and MCM6 messenger RNA, Ki67 positivity), along with an increase in keratinocyte migration. Using a small\u00a0interfering\u00a0RNA\u00a0mediated knockdown approach, we further demonstrated that miR\u201021\u20105p mediates its effects by suppressing the expression of programmed cell death 4 (PDCD4) and mammary serine protease inhibitor (Maspin), both tumor\u2010suppressor genes. Investigation of clinical samples corroborated the lower miR\u201021 levels and a higher expression of PDCD4 and Maspin in L Day 14 compared to the NL Day 14 epidermis. In conclusion, this study revealed that a relatively lower upregulation of miR\u201021\u20105p in L skin leads to significantly higher levels of PDCD4 and Maspin, delaying wound re\u2010epithelialization by reducing the proliferation and migration of keratinocytes.<\/jats:p>","DOI":"10.1002\/jcp.30614","type":"journal-article","created":{"date-parts":[[2021,10,23]],"date-time":"2021-10-23T05:51:52Z","timestamp":1634968312000},"page":"1429-1439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Differential regulation of miR\u201021\u20105p delays wound healing of melanocyte\u2010deprived vitiligo skin by modulating the expression of tumor\u2010suppressors PDCD4 and Maspin"],"prefix":"10.1002","volume":"237","author":[{"given":"Hemang D.","family":"Brahmbhatt","sequence":"first","affiliation":[{"name":"Skin Biology Laboratory CSIR\u2014Institute of Genomics and Integrative Biology New Delhi India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) Ghaziabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohit","family":"Gupta","sequence":"additional","affiliation":[{"name":"Skin Biology Laboratory CSIR\u2014Institute of Genomics and Integrative Biology New Delhi India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) Ghaziabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aayush","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Dr. D. 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