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N\u2010acetylglucosaminyltransferase V (GnT\u2010V) is the Golgi enzyme, and it has been reported that the \u03b21,6GlcNAc\u2010branched N\u2010linked glycans are associated with various cell behaviors. Therefore, we modify the gene expression of ACs by transfection of the GnT\u2010V\u2010overexpression plasmid, and we found that upregulation of GnT\u2010V extensively increased ACs proliferation and stemness properties in ACs\/GnT\u2010V compared to ACs transfected with Mock plasmid. More importantly, we observed that high levels of GnT\u2010V positively correlated with ALDH1A3 expression via increasing phosphorylation of cell surface receptors and activating the downstream signaling transduction. Hence, the current study suggested that GnT\u2010V is a significant factor for cell immortalization in the ACs model by activating the EGFR\/ERK\/ALDH1A3 signaling pathway.<\/jats:p>","DOI":"10.1002\/jcp.30641","type":"journal-article","created":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T06:57:39Z","timestamp":1637564259000},"page":"1780-1789","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Overexpression of N\u2010acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation"],"prefix":"10.1002","volume":"237","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1738-4242","authenticated-orcid":false,"given":"Ching\u2010Chia","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine and College of Engineering National Taiwan University Taipei 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