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Cell\u2010based therapies that stimulate these stromal components may be of therapeutic relevance for partially vital dental pulp conditions. This study aimed to examine the early effect of photobiomodulation (PBM) in pericytes from experimentally injured pulp tissue. To accomplish this, we used the Nestin\u2010GFP\/NG2\u2010DsRed mice, which\u00a0could allow the identification of distinct pericyte phenotypes. We discovered the presence of two pericytes subsets within the dental pulp, the Nestin <jats:sup>+<\/jats:sup>NG2<jats:sup>+<\/jats:sup> (type\u20102) and Nestin<jats:sup>\u2212<\/jats:sup>NG2<jats:sup>+<\/jats:sup> (type\u20101). Upon injury, PBM treatment led to a significant increase in Nestin<jats:sup>+<\/jats:sup> cells and pericytes. This boost was mainly conferred by the more committed pericyte subset (NestinNG2<jats:sup>+<\/jats:sup>). PBM also stimulated terminal blood vessels sprouting adjacent to the injury site while maintaining signs of pulp vitality. In vitro, PBM induced VEGF upregulation, improved dental pulp cells proliferation and migration, and favored their mineralization potential. Herein, different subsets of perivascular cells were unveiled in the pulp tissue. PBM enhanced not only NG2<jats:sup>+<\/jats:sup> cells but nestin\u2010expressing progenitors in the injured dental pulp.<\/jats:p>","DOI":"10.1002\/jcp.30680","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T07:18:36Z","timestamp":1642490316000},"page":"2198-2210","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Nestin and NG2 transgenes reveal two populations of perivascular cells stimulated by photobiomodulation"],"prefix":"10.1002","volume":"237","author":[{"given":"Nat\u00e1lia A.","family":"Gomes","sequence":"first","affiliation":[{"name":"Department of Restorative Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabella B.","family":"do Valle","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Surgery, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7847-9902","authenticated-orcid":false,"given":"Frederico O.","family":"Gleber\u2010Netto","sequence":"additional","affiliation":[{"name":"Department of Head &amp; Neck Surgery The University of Texas MD Anderson Cancer Center Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarc\u00edlia A.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Surgery, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hort\u00eancia M. de Castro","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Morphology, Biological Sciences Institute Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafaela F.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiza de Almeida Q.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lia S.","family":"Castilho","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo H. 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