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Damages to the corneal epithelium trigger important changes in the composition of the extracellular matrix to which the basal human corneal epithelial cells (hCECs) attach. These changes are perceived by membrane\u2010bound integrins and ultimately lead to re\u2010epithelialization of the injured epithelium through intracellular signalin. Among the many downstream targets of the integrin\u2010activated signaling pathways, WNK1 is the kinase whose activity is the most strongly increased during corneal wound healing. We previously demonstrated that pharmacological inhibition of WNK1 prevents proper closure of wounded human tissue\u2010engineered cornea in vitro. In the present study, we investigated the molecular mechanisms by which WNK1 contributes to corneal wound healing. By exploiting transcription factors microarrays, electrophoretic mobility\u2010shift assay, and gene profiling analyses, we demonstrated that the DNA binding properties and expression of numerous transcription factors (TFs), including the well\u2010known, ubiquitous TFs specific protein 1 (Sp1) and activator protein 1 (AP1), were reduced in hCECs upon WNK1 inhibition by WNK463. This process appears to be mediated at least in part by alteration in both the ubiquitination and glycosylation status of these TFs. These changes in TFs activity and expression impacted the transcription of several genes, including that encoding the \u03b15 integrin subunit, a well\u2010known target of both Sp1 and AP1. Gene profiling revealed that only a moderate number of genes in hCECs had their level of expression significantly altered in response to WNK463 exposition. Interestingly, analysis of the microarray data for these deregulated genes using the <jats:italic>ingenuity pathway analysis<\/jats:italic> software predicted that hCECs would stop migrating and proliferating but differentiate more when they are grown in the presence of the WNK1 inhibitor. These results demonstrate that WNK1 plays a critical function by orienting hCECs into the appropriate biological response during the process of corneal wound healing.<\/jats:p>","DOI":"10.1002\/jcp.30698","type":"journal-article","created":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T12:59:14Z","timestamp":1644670754000},"page":"2434-2450","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The WNK1 kinase regulates the stability of transcription factors during wound healing of human corneal epithelial cells"],"prefix":"10.1002","volume":"237","author":[{"given":"Pascale","family":"Desjardins","sequence":"first","affiliation":[{"name":"Centre Universitaire d'Ophtalmologie \u2010 Recherche (CUO\u2010Recherche) et Centre de Recherche du CHU de Qu\u00e9bec, Universit\u00e9 Laval  Qu\u00e9bec City Qu\u00e9bec Canada"},{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l'Universit\u00e9 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