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Since, LOX enzymes need copper (Cu) for their proper catalytic activity; the present study investigated the efficacy of a copper chelator, disulfiram (DSF) in renal fibrosis. Antifibrotic activity of DSF was investigated in kidney epithelial cells stimulated by transforming growth factor\u2010\u03b21 (5\u2009ng\/ml) as well as in two animal models. The renal injury was induced in animals by unilateral ureteral obstruction and folic acid administration (250\u2009mg\/kg). The DSF (3 and 10\u2009mg\/kg, every 3rd day) and standard LOX inhibitor, \u03b2\u2010aminopropionitrile (BAPN, 100\u2009mg\/kg, daily) administration was started on day 0 and continued till the day of sacrifice. DSF was found to be a potent LOX\/LOXL2 inhibitor to reduce crosslinking of collagen fibrils leading to reduction in the collagen deposition. In addition, the DSF was demonstrated to inhibit epithelial\u2010mesenchymal transition in the tubular cells and fibrotic kidneys. Our results suggested that DSF, being a clinically available drug could be translated to clinics for its potent antifibrotic activity due to its inhibitory effect on LOX proteins.<\/jats:p>","DOI":"10.1002\/jcp.30717","type":"journal-article","created":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T06:19:50Z","timestamp":1647238790000},"page":"2516-2527","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Disulfiram prevents collagen crosslinking and inhibits renal fibrosis by inhibiting lysyl oxidase enzymes"],"prefix":"10.1002","volume":"237","author":[{"given":"Mohd Aslam","family":"Saifi","sequence":"first","affiliation":[{"name":"Department of Biological Sciences (Regulatory Toxicology) National Institute of Pharmaceutical Education and Research (NIPER)  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arbaz 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