{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:16:47Z","timestamp":1787066207424,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T00:00:00Z","timestamp":1647820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["EuroTech Postdoc Programme (754462)"],"award-info":[{"award-number":["EuroTech Postdoc Programme (754462)"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["Leonardo da Vinci Grant (EAC\/S07\/2012)"],"award-info":[{"award-number":["Leonardo da Vinci Grant (EAC\/S07\/2012)"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We investigated whether 20 candidate single nucleotide polymorphisms (SNPs) were associated with in vivo exercise\u2010induced muscle damage (EIMD), and with an in vitro skeletal muscle stem cell wound healing assay. Sixty\u2010five young, untrained Caucasian adults performed 120 maximal eccentric knee\u2010extensions on an isokinetic dynamometer to induce EIMD. Maximal voluntary isometric\/isokinetic knee\u2010extensor torque, knee joint range of motion (ROM), muscle soreness, serum creatine kinase activity and interleukin\u20106 concentration were assessed before, directly after and 48\u2009h after EIMD. Muscle stem cells were cultured from\n                    <jats:italic>vastus lateralis<\/jats:italic>\n                    biopsies from a separate cohort (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912), and markers of repair were measured in vitro. Participants were genotyped for all 20 SNPs using real\u2010time PCR. Seven SNPs were associated with the response to EIMD, and these were used to calculate a total genotype score, which enabled participants to be segregated into three polygenic groups: \u2018preferential\u2019 (more \u2018protective\u2019 alleles), \u2018moderate\u2019, and \u2018non\u2010preferential\u2019. The non\u2010preferential group was consistently weaker than the preferential group (1.93\u2009\u00b1\u20090.81 vs. 2.73\u2009\u00b1\u20090.59\u2009N\u2009\u2219\u2009m\/kg;\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20099.51\u2009\u00d7\u200910\n                    <jats:sup>\u22124<\/jats:sup>\n                    ) and demonstrated more muscle soreness (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.011) and a larger decrease in knee joint ROM (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.006) following EIMD. Two\n                    <jats:italic>TTN\u2010AS1<\/jats:italic>\n                    SNPs in linkage disequilibrium were associated with in vivo EIMD (rs3731749,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.005) and accelerated muscle stem cell migration into the artificial wound in vitro (rs1001238,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.006). Thus, we have identified a polygenic profile, linked with both muscle weakness and poorer recovery following EIMD. Moreover, we provide evidence for a novel\n                    <jats:italic>TTN<\/jats:italic>\n                    gene\u2010cell\u2010skeletal muscle mechanism that may help explain some of the interindividual variability in the response to EIMD.\n                  <\/jats:p>","DOI":"10.1002\/jcp.30723","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T08:06:39Z","timestamp":1647849999000},"page":"2862-2876","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Polygenic mechanisms underpinning the response to exercise\u2010induced muscle damage in humans: In vivo and in vitro evidence"],"prefix":"10.1002","volume":"237","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5463-1954","authenticated-orcid":false,"given":"Philipp","family":"Baumert","sequence":"first","affiliation":[{"name":"Exercise Biology Group, Faculty of Sport and Health Sciences Technical University of Munich Munich Germany"},{"name":"School of Sport and Exercise Sciences Liverpool John Moores University Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Cocks","sequence":"additional","affiliation":[{"name":"School of Sport and Exercise Sciences Liverpool John Moores University Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliette A.","family":"Strauss","sequence":"additional","affiliation":[{"name":"School of Sport and Exercise Sciences Liverpool John Moores University Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam O.","family":"Shepherd","sequence":"additional","affiliation":[{"name":"School of Sport and Exercise Sciences Liverpool John Moores University Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry","family":"Drust","sequence":"additional","affiliation":[{"name":"School of Sport, Exercise and Rehabilitation Sciences University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Lake","sequence":"additional","affiliation":[{"name":"School of Sport and Exercise Sciences Liverpool John Moores University Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire E.","family":"Stewart","sequence":"additional","affiliation":[{"name":"School of Sport and Exercise Sciences Liverpool John Moores University Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5705-0207","authenticated-orcid":false,"given":"Robert M.","family":"Erskine","sequence":"additional","affiliation":[{"name":"School of Sport and Exercise Sciences Liverpool John Moores University Liverpool UK"},{"name":"Institute of Sport, Exercise and Health University College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715762.2014.928410"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ptsp.2014.04.004"},{"key":"e_1_2_10_4_1","article-title":"Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil\u2010Blakesley Conchotome","volume":"103","author":"Baczynska A. 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