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Nonsense <jats:italic>YAP1<\/jats:italic> mutations in humans result in autosomal dominant coloboma. Here, we generated a conditional knockout mouse model in which <jats:italic>Yap1<\/jats:italic> was specifically deleted in embryonic retinal progenitor cells (RPCs) and in mature M\u00fcller cells using a Chx10\u2010Cre driver. Our data demonstrated that the conditional ablation of <jats:italic>Yap1<\/jats:italic> in embryonic RPCs does not prevent normal retinal development and caused no gross changes in retinal structure during embryonic and early postnatal life. Nevertheless, <jats:italic>Yap1<\/jats:italic> deficient in retinal M\u00fcller cells in adult mice leads to impaired visual responses and extensive late\u2010onset retinal degeneration, characterized by reduced cell number in all retinal layers. Immunofluorescence data further revealed the degeneration and death of rod and cone photoreceptors, bipolar cells, horizontal cells, amacrine cells and ganglion cells to varying degrees in aged knockout mice. Moreover, alteration of glial homeostasis and reactive gliosis were also observed. Finally, cell proliferation and TUNEL assay revealed that the broad retinal degeneration is mainly caused by enhanced apoptosis in late period. Together, this work uncovers that YAP is essential for the normal vision and retinal maintenance, highlighting the crucial role of YAP in retinal function and homeostasis.<\/jats:p>","DOI":"10.1002\/jcp.30757","type":"journal-article","created":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T18:39:42Z","timestamp":1652121582000},"page":"2673-2689","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Specific ablation of Hippo signalling component <i>Yap1<\/i> in retinal progenitors and M\u00fcller cells results in late onset retinal degeneration"],"prefix":"10.1002","volume":"237","author":[{"given":"Yeming","family":"Yang","sequence":"first","affiliation":[{"name":"Sichuan Provincial Key Laboratory for Human Disease Gene Study, Center for Medical Genetics, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu Sichuan China"},{"name":"Department of Psychosomatic Medicine Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital Chengdu Sichuan China"},{"name":"Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences and Qinghai Provincial Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau Biology Xining Qinghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Sichuan Provincial Key Laboratory for Human Disease Gene Study, Center for Medical Genetics, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu Sichuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Li","sequence":"additional","affiliation":[{"name":"Sichuan Provincial Key Laboratory for Human Disease Gene Study, Center for Medical Genetics, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu Sichuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuanxiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Sichuan Provincial Key Laboratory for Human Disease Gene Study, Center for Medical Genetics, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu Sichuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2531-7552","authenticated-orcid":false,"given":"Xianjun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Sichuan Provincial Key Laboratory for Human Disease Gene Study, Center for Medical Genetics, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu Sichuan China"},{"name":"Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences and Qinghai Provincial Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau Biology Xining Qinghai China"},{"name":"Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026) Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital Chengdu Sichuan China"},{"name":"Departemnt of Ophthalmology First People's Hospital of Shangqiu Shangqiu Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Psychosomatic Medicine Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital Chengdu Sichuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097365"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-17994"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2008.10.014"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2006.05.003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000328979"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076075"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.08488"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2015.10"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.2.589"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.07.019"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/78774"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2015.00484"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddh106"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.16-21366"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/msb.201304750"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.311950"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2018.0910"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6989(85)90214-7"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901340304"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-14774-2"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-21-08936.1998"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.04.070"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10703"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2016.09.001"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2018.07.020"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24628"},{"key":"e_1_2_7_28_1","first-page":"1310","article-title":"Cre\u2010mediated recombination efficiency and transgene expression patterns of three retinal bipolar cell\u2010expressing Cre transgenic mouse lines","volume":"19","author":"Lu Q.","year":"2013","journal-title":"Molecular Vision"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201503115"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-02860-9"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.274027.115"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119008"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.47.3.445"},{"issue":"4","key":"e_1_2_7_34_1","first-page":"257","article-title":"Hippo signaling circuit and divergent tissue growth in mammalian eye","volume":"41","author":"Moon K. 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