{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:03:41Z","timestamp":1791407021993,"version":"4.3.3"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers because of its late diagnosis and chemoresistance. Primary cilia, the cellular antennae, are observed in most human cells to maintain development and differentiation. Primary cilia are gradually lost during the progression of pancreatic cancer and are eventually absent in PDAC. Here, we showed that cisplatin\u2010resistant PDAC regrew primary cilia. Additionally, genetic or pharmacological disruption of primary cilia sensitized PDAC to cisplatin treatment. Mechanistically, ataxia telangiectasia mutated (ATM) and ATM and RAD3\u2010related (ATR), tumor suppressors that initiate DNA damage responses, promoted the excessive formation of centriolar satellites (EFoCS) and autophagy activation. Disruption of EFoCS and autophagy inhibited primary ciliogenesis, sensitizing PDAC cells to cisplatin treatment. Collectively, our findings revealed an unexpected interplay among the DNA damage response, primary cilia, and chemoresistance in PDAC and deciphered the molecular mechanism by which ATM\/ATR\u2010mediated EFoCS and autophagy cooperatively regulate primary ciliogenesis.<\/jats:p>","DOI":"10.1002\/jcp.30898","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T15:16:05Z","timestamp":1666019765000},"page":"4487-4503","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["ATM\u2010 and ATR\u2010induced primary ciliogenesis promotes cisplatin resistance in pancreatic ductal adenocarcinoma"],"prefix":"10.1002","volume":"237","author":[{"given":"Yu\u2010Ying","family":"Chao","sequence":"first","affiliation":[{"name":"Institute of Basic Medical Sciences, College of Medicine National Cheng Kung University Tainan Taiwan"},{"name":"Department of Cell Biology and Anatomy National Cheng Kung 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