{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:18:35Z","timestamp":1740104315788,"version":"3.37.3"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T00:00:00Z","timestamp":1682467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Binding of lipopolysaccharide (LPS) to CD14 is required for its cellular effects via TLR4. A role of LPS\/TLR4\u2010mediated signaling in activated hepatic stellate cells (aHSCs), the major fibrogenic cells, in liver fibrosis has been reported. We investigated effects of LPS on carbon tetrachloride (CCl4)\u2010induced fibrosis in CD14\u2010knockout (KO) mice <jats:italic>in vivo<\/jats:italic>, and culture\u2010activated HSCs in vitro. CCl4\u00a0(biweekly; 4 weeks)\u2010treated wild type (WT) and CD14\u2010KO mice were challenged with single LPS administration for 24\u2009h. Liver injury, inflammation and fibrosis were determined. Culture\u2010activated HSCs from WT or CD14\u2010KO mice were stimulated with LPS. Parameters of fibrogenic activity (expression of collagen1a1 [Col1a1], \u03b1\u2010smooth muscle actin [\u03b1SMA] and TGF\u03b21) and inflammatory cytokines\/chemokines were measured. CCl4 treatment caused similar liver injury and fibrosis in WT and CD14\u2010KO mice. LPS increased liver injury and inflammation similarly in CCl4\u2010treated WT and CD14\u2010KO mice, but downregulated <jats:italic>Timp1<\/jats:italic> and upregulated <jats:italic>Mmp13<\/jats:italic>. LPS elicited similar NF\u03baB activation and inflammatory response in WT and CD14\u2010KO aHSCs. LPS similarly downregulated <jats:italic>Acta2<\/jats:italic> (encodes \u03b1SMA), <jats:italic>Pdgfrb, Col1a1<\/jats:italic> and <jats:italic>Mmp13<\/jats:italic> expression but did not affect <jats:italic>Timp1<\/jats:italic> expression in WT and CD14\u2010KO aHSCs. LPS did not alter <jats:italic>Tgfb1<\/jats:italic> but increased expression of decorin (<jats:italic>Dcn<\/jats:italic>) (inhibitor of TGF\u03b21) expression in WT and CD14\u2010KO aHSCs. The results indicate that the effects of LPS on HSCs are CD14\u2010independent, and CD14 is not required for hepatic fibrosis. LPS\u2010induced down\u2010modulation of fibrogenic markers in aHSCs is also CD14\u2010independent.<\/jats:p>","DOI":"10.1002\/jcp.31030","type":"journal-article","created":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T12:38:24Z","timestamp":1682512704000},"page":"1530-1541","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["CD14 is not required for carbon tetrachloride\u2010induced hepatic inflammation and fibrosis with or without lipopolysaccharide challenge"],"prefix":"10.1002","volume":"238","author":[{"given":"Akanksha","family":"Sharma","sequence":"first","affiliation":[{"name":"Cincinnati Veterans Administration Medical Center Cincinnati Ohio USA"},{"name":"Cincinnati Children's Hospital Medical Center Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Cincinnati Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9127-9518","authenticated-orcid":false,"given":"Chandrashekhar R.","family":"Gandhi","sequence":"additional","affiliation":[{"name":"Cincinnati Veterans Administration Medical Center Cincinnati Ohio USA"},{"name":"Cincinnati Children's Hospital Medical Center Cincinnati Ohio USA"},{"name":"Department of Surgery University of Cincinnati Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,4,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2010.172"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155412438104"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v110.pm8608892"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15632"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800134-9.00007-5"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ALC.0000171977.25531.7A"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200522675"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-005-1055-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2021.01.002"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.8.5288"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00013.2007"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2017.06.001"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2020.00130"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082159"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800134-9.00004-X"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80254-X"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.11.7269-7272.2005"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.24236"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.149.3.613"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22950"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1240248"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2017.01.021"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-259515"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.8.4272"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.10.003"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2013.97"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25567"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1119964109"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2021.11.011"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.975027"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00182"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.01.038"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1663"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.31188"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13683"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21254"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704151"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(99)80004-1"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00032"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.31030","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T12:18:36Z","timestamp":1692274716000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.31030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,26]]},"references-count":39,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1002\/jcp.31030"],"URL":"https:\/\/doi.org\/10.1002\/jcp.31030","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"type":"print","value":"0021-9541"},{"type":"electronic","value":"1097-4652"}],"subject":[],"published":{"date-parts":[[2023,4,26]]},"assertion":[{"value":"2022-10-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}