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Yet, the mechanism remains unknown. Cases of spotted fever rickettsioses have been increasing, and infections with these bacteria cause life\u2010threatening diseases by targeting brain and lung tissues. Therefore, the goal of the present study is to further dissect the molecular mechanism underlying\n                    <jats:italic>R<\/jats:italic>\n                    \u2010ECExos\u2010induced barrier dysfunction of normal recipient microvascular endothelial cells (MECs), depending on their exosomal RNA cargos. Infected ticks transmit the rickettsiae to human hosts following a bite and injections of the bacteria into the skin. In the present study, we demonstrate that treatment with\n                    <jats:italic>R<\/jats:italic>\n                    \u2010ECExos, which were derived from spotted fever group\n                    <jats:italic>R parkeri<\/jats:italic>\n                    infected human dermal MECs, induced disruptions of the paracellular adherens junctional protein VE\u2010cadherin, and breached the paracellular barrier function in recipient pulmonary MECs (PMECs) in an exosomal RNA\u2010dependent manner. We did not detect different levels of miRs in parent dermal MECs following rickettsial infections. However, we demonstrated that the microvasculopathy\u2010relevant miR23a\u201027a\u201024 cluster and miR30b are selectively enriched in\n                    <jats:italic>R<\/jats:italic>\n                    \u2010ECExos. Bioinformatic analysis revealed that common sequence motifs are shared exclusively among the exosomal, selectively\u2010enriched miR23a cluster and miR30b at different levels. Taken together, these data warrant further functional identification and characterization of a monopartition, bipartition, or tripartition among ACA, UCA, and CAG motifs that guide recognition of microvasculopathy\u2010relevant miR23a\u201027a\u201024 and miR30b, and subsequently results in their selective enrichments in\n                    <jats:italic>R<\/jats:italic>\n                    \u2010ECExos.\n                  <\/jats:p>","DOI":"10.1002\/jcp.31061","type":"journal-article","created":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T07:13:30Z","timestamp":1687158810000},"page":"1937-1948","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification of common sequence motifs shared exclusively among selectively packed exosomal pathogenic microRNAs during rickettsial 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