{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T01:49:48Z","timestamp":1785376188114,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T00:00:00Z","timestamp":1687651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It has been known that periodontal ligament\u2010associated protein\u20101 (PLAP\u20101\/Asporin) not only inhibits cartilage formation in osteoarthritis, but it also influences the healing of skull defect. However, the effect and mechanism of PLAP\u20101\/Asporin on the mutual regulation of osteoclasts and osteoblasts in periodontitis are not clear. In this study, we utilized a <jats:italic>PLAP\u20101\/Asporin<\/jats:italic> gene knockout (KO) mouse model to research this unknown issue. We cultured mouse bone marrow mesenchymal stem cells with <jats:italic>Porphyromonas gingivalis<\/jats:italic> lipopolysaccharide (<jats:italic>P.g<\/jats:italic>. LPS) for osteogenic induction in vitro. The molecular mechanism of PLAP\u20101\/Asporin in the regulation of osteoblasts was detected by immunoprecipitation, immunofluorescence, and inhibitors of signaling pathways. The results showed that the KO of <jats:italic>PLAP\u20101\/Asporin<\/jats:italic> promoted osteogenic differentiation through transforming growth factor beta 1 (TGF\u2010\u03b21)\/Smad3 in inflammatory environments. We further found the KO of <jats:italic>PLAP\u20101\/Asporin<\/jats:italic> inhibited osteoclast differentiation and promoted osteogenic differentiation through the TGF\u2010\u03b21\/Smad signaling pathway in an inflammatory coculture system. The experimental periodontitis model was established by silk ligation and the alveolar bone formation in <jats:italic>PLAP\u20101\/Asporin<\/jats:italic> KO mice was promoted through TGF\u2010\u03b21\/Smad3 signaling pathway. The subcutaneous osteogenesis model in nude mice also confirmed that the KO of <jats:italic>PLAP\u20101\/Asporin<\/jats:italic> promoted bone formation by the histochemical staining. In conclusion, PLAP\u20101\/Asporin regulated the differentiation of osteoclasts and osteoblasts through TGF\u2010\u03b21\/Smad signaling pathway. The results of this study lay a theoretical foundation for the further study of the pathological mechanism underlying alveolar bone resorption, and the prevention and treatment of periodontitis.<\/jats:p>","DOI":"10.1002\/jcp.31062","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T04:46:11Z","timestamp":1687754771000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Periodontal ligament\u2010associated protein\u20101 knockout mice regulate the differentiation of osteoclasts and osteoblasts through TGF\u2010\u03b21\/Smad signaling pathway"],"prefix":"10.1002","volume":"239","author":[{"given":"Shuang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration &amp; Shandong Provincial Clinical Research Center for Oral Diseases Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration &amp; Shandong Provincial Clinical Research Center for Oral Diseases Jinan Shandong China"},{"name":"Department of Stomatology the Second Hospital of Shandong University Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration &amp; Shandong Provincial Clinical Research Center for Oral Diseases Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"An","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration &amp; Shandong Provincial Clinical Research Center for Oral Diseases Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingrui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration &amp; Shandong Provincial Clinical Research Center for Oral Diseases Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; 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