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However, TNF\u2010\u03b1\u00a0regulating macrophage polarization in liver injury induced by TCE is still unknown. Thus, on the basis of our previous research, we established the TCE\u2010sensitized BALB\/c mouse model with R7050, a specific inhibitor of TNFR1. Then, we observed significant decreases in autophagy related protein and gene levels in M1 macrophage in TCE positive group, and R7050 can relieve M1 macrophage autophagy. We also found the phosphorylated form of mammalian target of Rapamycin (mTOR) was activated and the expression of p\u2010mTOR\u00a0protein increased induce by TCE. In vitro, we found TNFR1 and CD11c were increased in RAW264.7\u00a0cell line with TNF\u2010\u03b1.\u00a0And then we use Zafirlukast (Zaf), an TNFR1 antagonist, CD11c and TNFR1 reduced significantly, we also found p\u2010mTOR\u00a0expression increased after TNF\u2010\u03b1\u00a0treatment, but decreased in TNF\u2010\u03b1\u00a0+ Zaf group. Further, we used Rapamycin (RAP),\u00a0a mTOR\u2010specific inhibitor, to establish a TCE\u2010sensitized mice model and found the expression levels of p62 and p\u2010mTOR\u00a0proteins increased and LC3B decreased in the TCE positive group, while RAP treatment reversed the trends of all of these proteins. Rapamycin prevented the TNF\u2010\u03b1\u2010induced p\u2010mTOR increase and dramatically downregulated IL\u20101\u03b2 expression in the RAW264.7\u00a0cell line with TNF\u2010\u03b1\u00a0treatment. The results uncover a novel role for TNF\u2010\u03b1\/TNFR1,\u00a0which promotes M1 polarization of macrophage and suppresses macrophage autophagy via the mTOR pathway.<\/jats:p>","DOI":"10.1002\/jcp.31083","type":"journal-article","created":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T16:03:36Z","timestamp":1690301016000},"page":"2267-2281","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Macrophage autophagy contributes to immune liver injury in trichloroethylene sensitized mice: Critical role of TNF\u2010\u03b1 mediating mTOR pathway"],"prefix":"10.1002","volume":"238","author":[{"given":"Baiwang","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Occupational Health and Environmental Health, School of Public Health Anhui Medical University Hefei Anhui 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