{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:24:53Z","timestamp":1789579493855,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T00:00:00Z","timestamp":1691539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gastrointestinal epithelial cells respond to milk\u2010born molecules throughout breastfeeding, influencing growth, and development. The rapid renewal of the small intestine depends on the proliferation in the crypt that drives cell fates. We used early weaning model to investigate immediate and late effects of breastfeeding on proliferation, differentiation of jejunal epithelial cells. Wistar rats were either allowed to suckle (S) until 21 postnatal days or submitted to early weaning (EW) at 15 days. By comparing ages (18, 60, and 120 days), we found that EW decreased Ki67 indices and villi height at 18 and 60 days (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), and at 120 days they were similar between diets. Proliferative reduction and augmented expression of <jats:italic>Cdkn1b<\/jats:italic> (p27 gene) were parallel. In the stem cell niche, EW increased the number and activity (<jats:italic>Defa24<\/jats:italic>) of Paneth cells at 18 and 60 days (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), and <jats:italic>Lgr5<\/jats:italic> and <jats:italic>Ascl2 genes<\/jats:italic> showed inverted responses between ages. Among target cells, EW decreased goblet cell number at 18 and 60 days (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and increased it at 120 days (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), whereas enteroendocrine marker genes were differentially altered. EW reduced enterocytes density at 18 days (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), and at 120 days this population was decreased (vs. 60 days). Among cell fate crypt\u2010controlling genes, <jats:italic>Notch<\/jats:italic> and <jats:italic>Atoh1<\/jats:italic> were the main targets of EW. Metabolically, intraperitoneal glucose tolerance was immediately reduced (18 days), being reverted until 120 days (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Currently, we showed that breastfeeding has a lifespan influence on intestinal mucosa and on its stem cell compartment. We suggest that, although jejunum absorptive function is granted after early weaning, the long lasting changes in gene expression might prime the mucosa with a different sensitivity to gut disorders that still have to be further explored.<\/jats:p>","DOI":"10.1002\/jcp.31089","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T11:37:24Z","timestamp":1691581044000},"page":"2304-2315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Breastfeeding lifespan control of growth, maintenance, and metabolism of small intestinal epithelium"],"prefix":"10.1002","volume":"238","author":[{"given":"Aline Vasques da","family":"Costa","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology Institute of Biomedical Sciences, University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isadora Campos","family":"Rattes","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Institute of Biomedical Sciences, University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Purcell","family":"Goes","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Institute of Biomedical Sciences, University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa Honda Greco","family":"Lobo","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Institute of Biomedical Sciences, University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laylla Barreto e","family":"Barreto","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Institute of Biomedical Sciences, University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1863-893X","authenticated-orcid":false,"given":"Patricia","family":"Gama","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Institute of Biomedical Sciences, University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1977.234"},{"issue":"8","key":"e_1_2_9_3_1","first-page":"869","article-title":"Fasting enhances cell proliferation of gastric epithelium during the suckling period in rats","volume":"26","author":"Alvares E. 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