{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:01:08Z","timestamp":1791316868857,"version":"4.3.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T00:00:00Z","timestamp":1705017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2019R1A2C1003398"],"award-info":[{"award-number":["2019R1A2C1003398"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1A6A3A01086647"],"award-info":[{"award-number":["2021R1A6A3A01086647"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Obesity and metabolic disorders caused by alterations in lipid metabolism are major health issues in developed, affluent societies. Adipose tissue is the only organ that stores lipids and prevents lipotoxicity in other organs. Mature adipocytes can affect themselves and distant metabolism\u2010related tissues by producing various adipokines, including adiponectin and leptin. The engulfment adaptor phosphotyrosine\u2010binding domain\u2010containing 1 (GULP1) regulates intracellular trafficking of glycosphingolipids and cholesterol, suggesting its close association with lipid metabolism. However, the role of GULP1 in adipocytes remains unknown. Therefore, this study aimed to investigate the function of GULP1 in adipogenesis, glucose uptake, and the insulin signaling pathway in adipocytes. A 3T3\u2010L1 cell line with <jats:italic>Gulp1<\/jats:italic> knockdown (shGulp1) and a 3T3\u2010L1 control group (U6) were established. Changes in shGulp1 cells due to GULP1 deficiency were examined and compared to those in U6 cells using microarray analysis. Glucose uptake was monitored via insulin stimulation in shGulp1 and U6 cells using a 2\u2010NBDG glucose uptake assay, and the insulin signaling pathway was investigated by western blot analysis. Adipogenesis was significantly delayed, lipid metabolism was altered, and several adipogenesis\u2010related genes were downregulated in shGulp1 cells compared to those in U6 cells. Microarray analysis revealed significant inhibition of peroxisome proliferator\u2010activated receptor signaling in shGulp1 cells compared with U6 cells. The production and secretion of adiponectin as well as the expression of adiponectin receptor were decreased in shGulp1 cells. In particular, compared with U6 cells, glucose uptake via insulin stimulation was significantly decreased in shGulp1 cells through the disturbance of ERK1\/2 phosphorylation. This is the first study to identify the role of GULP1 in adipogenesis and insulin\u2010stimulated glucose uptake by adipocytes, thereby providing new insights into the differentiation and functions of adipocytes and the metabolism of lipids and glucose, which can help better understand metabolic diseases.<\/jats:p>","DOI":"10.1002\/jcp.31173","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T09:14:56Z","timestamp":1705050896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["GULP1 deficiency reduces adipogenesis and glucose uptake via downregulation of PPAR signaling and disturbing of insulin\/ERK signaling in 3T3\u2010L1 cells"],"prefix":"10.1002","volume":"239","author":[{"given":"Soon\u2010Young","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine, Cell and Matrix Research Institute, School of Medicine Kyungpook National University Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung\u2010Yoon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine Dongguk University Gyeongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3342-8359","authenticated-orcid":false,"given":"Jung\u2010Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Cell and Matrix Research Institute, School of Medicine Kyungpook National University Daegu Republic of Korea"},{"name":"Department of Biomedical Science, BK21 Four KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents Kyungpook National University Daegu Republic of 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