{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T14:41:30Z","timestamp":1783435290357,"version":"3.54.6"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T00:00:00Z","timestamp":1705017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Carotid body tumor (CBT) is a rare neck tumor located at the adventitia of the common carotid artery bifurcation. The prominent pathological features of CBT are high vascularization and abnormal proliferation. However, single\u2010cell transcriptome analysis of the microenvironment composition and molecular complexity in CBT has yet to be performed. In this study, we performed single\u2010cell RNA sequencing (scRNA\u2010seq) analysis on human CBT to define the cells that contribute to hypervascularization and chronic hyperplasia. Unbiased clustering analysis of transcriptional profiles identified 16 distinct cell populations including endothelial cells (ECs), smooth muscle cells (SMCs), neuron cells, macrophage cells, neutrophil cells, and T cells. Within the ECs population, we defined subsets with angiogenic capacity plus clear signs of later endothelial progenitor cells (EPCs) to normal ECs. Two populations of macrophages were detectable in CBT, macrophage1 showed enrichment in hypoxia\u2010inducible factor\u20101 (HIF\u20101) and as well as an early EPCs cell\u2010like population expressing CD14 and vascular endothelial growth factor. In addition to HIF\u20101\u2010related transcriptional protein expression, macrophages1 also display a neovasculogenesis\u2010promoting phenotype. SMCs included three\u00a0populations showing platelet\u2010derived growth factor receptor beta\u00a0and vimentin\u00a0expression, indicative of a cancer\u2010associated fibroblast\u00a0phenotype. Finally, we identified three types of neuronal cells, including chief cells and sustentacular cells, and elucidated their distinct roles in the pathogenesis of CBT and abnormal proliferation of tumors. Overall, our study provided the first comprehensive characterization of the transcriptional landscape of CBT at scRNA\u2010seq profiles, providing novel insights into the mechanisms underlying its formation.<\/jats:p>","DOI":"10.1002\/jcp.31175","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T04:35:40Z","timestamp":1705034140000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Single\u2010cell transcriptome analysis reveals tumoral microenvironment heterogenicity and hypervascularization in human carotid body tumor"],"prefix":"10.1002","volume":"239","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2787-4131","authenticated-orcid":false,"given":"Gaopo","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Endovascular Surgery The First Affiliated Hospital of Zhengzhou University Zhengzhou 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