{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T16:27:25Z","timestamp":1785342445025,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Interleukin\u201038\u00a0(IL\u201038), recently recognized as a cytokine with anti\u2010inflammatory properties that mitigate type 2 diabetes, has been associated with indicators of insulin resistance and nonalcoholic fatty liver disease (NAFLD). This study investigated the impact of IL\u201038 on hepatic lipid metabolism and endoplasmic reticulum (ER) stress. We assessed protein expression levels using Western blot analysis, while monodansylcadaverine\u00a0staining was employed to detect autophagosomes in hepatocytes. Oil red O staining was utilized to examine lipid deposition. The study revealed elevated serum IL\u201038 levels in high\u2010fat diet (HFD)\u2010fed mice and IL\u201038 secretion from mouse keratinocytes. IL\u201038 treatment attenuated lipogenic lipid accumulation and ER stress markers in hepatocytes exposed to palmitate. Furthermore, IL\u201038 treatment increased AMP\u2010activated protein kinase (AMPK) phosphorylation and autophagy. The effects of IL\u201038 on lipogenic lipid deposition and ER stress were nullified in cultured hepatocytes by suppressing AMPK through small interfering (si) RNA or 3\u2010methyladenine (3MA). In animal studies, IL\u201038 administration mitigated hepatic steatosis by suppressing the expression of lipogenic proteins and ER stress markers while reversing AMPK phosphorylation and autophagy markers in the livers of HFD\u2010fed mice. Additionally, AMPK siRNA, but not 3MA, mitigated IL\u201038\u2010enhanced fatty acid oxidation in hepatocytes. In summary, IL\u201038 alleviates hepatic steatosis through AMPK\/autophagy signaling\u2010dependent attenuation of ER stress and enhancement of fatty acid oxidation via the AMPK pathway, suggesting a therapeutic strategy for treating NAFLD.<\/jats:p>","DOI":"10.1002\/jcp.31184","type":"journal-article","created":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T06:50:42Z","timestamp":1704869442000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Interleukin\u201038 alleviates hepatic steatosis through AMPK\/autophagy\u2010mediated suppression of endoplasmic reticulum stress in obesity models"],"prefix":"10.1002","volume":"239","author":[{"given":"Jaw Long","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Pharmacology Chung\u2010Ang University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wonjun","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Chung\u2010Ang University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heeseung","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Chung\u2010Ang University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7907","authenticated-orcid":false,"given":"A. 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