{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T06:28:30Z","timestamp":1770532110558,"version":"3.49.0"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>With the prevalence of coronavirus disease 2019, the administration of glucocorticoids (GCs) has become more widespread. Treatment with high\u2010dose GCs leads to a variety of problems, of which steroid\u2010induced osteonecrosis of the femoral head (SONFH) is the most concerning. Since hypoxia\u2010inducible factor 1\u03b1 (HIF\u20101\u03b1) is a key factor in cartilage development and homeostasis, it may play an important role in the development of SONFH. In this study, SONFH models were established using methylprednisolone (MPS) in mouse and its proliferating chondrocytes to investigate the role of HIF\u20101\u03b1 in cartilage differentiation, extracellular matrix (ECM) homeostasis, apoptosis and glycolysis in SONFH mice. The results showed that MPS successfully induced SONFH in vivo and vitro, and MPS\u2010treated cartilage and chondrocytes demonstrated disturbed ECM homeostasis, significantly increased chondrocyte apoptosis rate and glycolysis level. However, compared with normal mice, not only the expression of genes related to collagens and glycolysis, but also chondrocyte apoptosis did not demonstrate significant differences in mice co\u2010treated with MPS and HIF\u20101\u03b1 inhibitor. And the effects observed in HIF\u20101\u03b1 activator\u2010treated chondrocytes were similar to those induced by MPS. And HIF\u20101\u03b1 degraded collagens in cartilage by upregulating its downstream target genes matrix metalloproteinases. The results of activator\/inhibitor of endoplasmic reticulum stress (ERS) pathway revealed that the high apoptosis rate induced by MPS was related to the ERS pathway, which was also affected by HIF\u20101\u03b1. Furthermore, HIF\u20101\u03b1 affected glucose metabolism in cartilage by increasing the expression of glycolysis\u2010related genes. In conclusion, HIF\u20101\u03b1 plays a vital role in the pathogenesis of SONFH by regulating ECM homeostasis, chondrocyte apoptosis, and glycolysis.<\/jats:p>","DOI":"10.1002\/jcp.31224","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T05:31:30Z","timestamp":1710394290000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["HIF\u20101\u03b1 in cartilage homeostasis, apoptosis, and glycolysis in mice with steroid\u2010induced osteonecrosis of the femoral head"],"prefix":"10.1002","volume":"239","author":[{"given":"Yaling","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Science, College of Veterinary Medicine Nanjing Agricultural University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Science, College 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