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To directly and better understand the protection of Gpx5 against OS in epididymal cells and sperm, we studied its mechanism of antioxidant protection from multiple aspects. To more directly investigate the role of Gpx5 in combating oxidative damage, we started with epididymal tissue morphology and Gpx5 expression profiles in combination with the mouse epididymal epithelial cell line PC1 (proximal caput 1) expressing recombinant Gpx5. The Gpx5 is highly expressed in adult male epididymal caput, and its protein signal can be detected in the sperm of the whole epididymis. Gpx5 has been shown to alleviate OS damage induced by 3\u2010Nitropropionic Acid (3\u2010NPA), including enhancing antioxidant activity, reducing mitochondrial damage, and suppressing cell apoptosis. Gpx5 reduces OS damage in PC1 and maintains the well\u2010functioning extracellular vesicles (EVs) secreted by PC1, and the additional epididymal EVs play a role in the response of sperm to OS damage, including reducing plasma membrane oxidation and death, and increasing sperm motility and sperm\u2010egg binding ability. Our study suggests that GPX5 plays an important role as an antioxidant in the antioxidant processes of epididymal cells and sperm, including plasma membrane oxidation, mitochondrial oxidation, apoptosis, sperm motility, and sperm\u2010egg binding ability.<\/jats:p>","DOI":"10.1002\/jcp.31273","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T09:20:02Z","timestamp":1714123202000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Exploring novel function of Gpx5 antioxidant activity: Assisting epididymal cells secrete functional extracellular vesicles"],"prefix":"10.1002","volume":"239","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9484-8579","authenticated-orcid":false,"given":"Long","family":"Li","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianqi","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoxian","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heran","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuzi","family":"Fang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qimin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dwi","family":"Wijayanti","sequence":"additional","affiliation":[{"name":"Department of Animal Science Perjuangan University of Tasikmalaya Tasikmalaya West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuzi","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-015-0032-1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.190055"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1150001"},{"key":"e_1_2_9_5_1","first-page":"1849","article-title":"Gpx5 protects the family jewels","volume":"119","author":"Aitken R. 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