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G protein\u2010coupled receptor 124 (GPR124) is significantly expressed at various stages of the human reproductive process, particularly during embryogenesis and angiogenesis. Our prior investigation demonstrated a notable decrease in GPR124 expression in the placentas of patients with early\u2010onset preeclampsia compared to that in normal pregnancy placentas. However, there is a lack of extensive investigation into the molecular processes that contribute to the role of GPR124 in placenta development. This study aimed to examine the mechanisms by which GPR124 affects the occurrence of early\u2010onset preeclampsia and its function in trophoblast. Proliferative, invasive, migratory, apoptotic, and inflammatory processes were identified in GPR124 knockdown, GPR124 overexpression, and normal HTR8\/SVneo cells. The mechanism of GPR124\u2010mediated cell function in GPR124 knockdown HTR8\/SVneo cells was examined using inhibitors of the JNK or P38 MAPK pathway. Downregulation of GPR124 was found to significantly inhibit proliferation, invasion and migration, and promote apoptosis of HTR8\/SVneo cells when compared to the control and GPR124 overexpression groups. This observation is consistent with the pathological characteristics of preeclampsia. In addition, GPR124 overexpression inhibits the secretion of pro\u2010inflammatory cytokines interleukin (IL)\u20108 and interferon\u2010\u03b3 (IFN\u2010\u03b3) while enhancing the secretion of the anti\u2010inflammatory cytokine interleukin (IL)\u20104. Furthermore, GPR124 suppresses the activation of P\u2010JNK and P\u2010P38 within the JNK\/P38 MAPK pathway. The invasion, apoptosis, and inflammation mediated by GPR124 were partially restored by suppressing the JNK and P38 MAPK pathways in HTR8\/SVneo cells. GPR124 plays a crucial role in regulating trophoblast proliferation, invasion, migration, apoptosis, and inflammation via the JNK and P38 MAPK pathways. Furthermore, the effect of GPR124 on trophoblast suggests its involvement in the pathogenesis of early\u2010onset preeclampsia.<\/jats:p>","DOI":"10.1002\/jcp.31298","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T06:29:45Z","timestamp":1716186585000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["GPR124 promotes trophoblast proliferation, migration, and invasion and inhibits trophoblast cell apoptosis and inflammation via JNK and P38 MAPK pathways"],"prefix":"10.1002","volume":"239","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4383-6903","authenticated-orcid":false,"given":"Yan","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Shenzhen Maternity and Child Healthcare Hospital, Cheeloo College of Medicine Shandong University shenzhen Guangdong China"},{"name":"Department of Obstetrics, Shandong Provincial Maternal and Child Health Care 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Sun Yat\u2010Sen University Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyu","family":"Cui","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital Jinan 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