{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T15:33:41Z","timestamp":1786376021775,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T00:00:00Z","timestamp":1720569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Piezo1 is a Ca<jats:sup>2+<\/jats:sup>\u2010permeable mechanically activated ion channel that is involved in various physiological processes and cellular responses to mechanical stimuli. The study of biophysical characteristics of Piezo1 is important for understanding the mechanisms of its function and regulation. Stretch activation, a routine approach that is applied to stimulate Piezo1 activity in the plasma membrane, has a number of significant limitations that complicate precise single\u2010channel analysis. Here, we aimed to determine pore properties of native Piezo1, specifically to examine permeation for physiologically relevant signaling divalent ions (calcium and magnesium) in human myeloid leukemia K562 cells using Piezo1\u2010specific chemical agonist, Yoda1. Using a combination of low\u2010noise single\u2010current patch\u2010clamp recordings of Piezo1 activity in response to Yoda1, we have determined single\u2010channel characteristics of native Piezo1 under various ionic conditions. Whole\u2010cell assay allowed us to directly measure Piezo1 single currents carried by Ca<jats:sup>2+<\/jats:sup> or Mg<jats:sup>2+<\/jats:sup> ions in the absence of other permeable cations in the extracellular solutions; unitary conductance values estimated at various concentrations of Mg<jats:sup>2+<\/jats:sup> revealed strong saturation effect. Patch clamp data complemented with fluorescent imaging clearly evidenced Ca<jats:sup>2+<\/jats:sup> and Mg<jats:sup>2+<\/jats:sup> entry via native Piezo1 channel in human leukemia K562 cells. Mg<jats:sup>2+<\/jats:sup> influx via Piezo1 was detected under quasi\u2010physiological conditions, thus showing that Piezo1 channels could potentially provide the physiological relevant pathway for Mg<jats:sup>2+<\/jats:sup> ion transport and contribute to the regulation of Mg<jats:sup>2+<\/jats:sup>\u2010dependent intracellular signaling.<\/jats:p>","DOI":"10.1002\/jcp.31371","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T06:05:42Z","timestamp":1720677942000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Functional characterization of native Piezo1 as calcium and magnesium influx pathway in human myeloid leukemia cells"],"prefix":"10.1002","volume":"239","author":[{"given":"Valeria Y.","family":"Vasileva","sequence":"first","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences St. Petersburg 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