{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T19:14:14Z","timestamp":1787944454567,"version":"build-2784847793"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,6]],"date-time":"2024-10-06T00:00:00Z","timestamp":1728172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>S\u2010adenosylmethionine (SAM) as a major methyl donor plays a key role in methylation modification in vivo, and its disorder was closely related to neural tube defects (NTDs). However, the exact mechanism between SAM deficiency and NTDs remained unclearly. Hence, we investigated the association between histone methylation modification and cell differentiation in NTDs mice induced by SAM deficiency. The levels of SAM and SAH (S\u2010adenosylhomocysteine) were determined by enzyme linked immunosorbent assay (ELISA). The level of histone methylation, \u03b2\u2010catenin were analyzed by Western blot, reversing transcription and quantitative PCR (RT\u2010qPCR) and immunofluorescence. The results showed that the incidence rate of NTDs induced by ethionine were 46.2%. Post treatment of ethionine combined with SAM, the incidence rate of NTDs was reduced to 26.2%. The level of SAM was significantly decreased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and a reduction in the SAM\/SAH ratio was observed after entionine treatment. The SAM deficiency caused the reduction of H3K27me3 modifications and the elevated UTX activity (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), and inhibited the expressions of \u03b2\u2010catenin. The differentiations of NSCs into neurons and oligodendrocytes were inhibited under SAM deficiency (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). These results indicated that the SAM deficiency led to reduce H3K27me3 modifications, prevented the \u03b2\u2010catenin signaling pathway and NSCs differentiation, which provided an understanding of the novel function of epigenetic regulation in NTDs.<\/jats:p>","DOI":"10.1002\/jcp.31452","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T05:25:07Z","timestamp":1728278707000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Ethionine\u2010induced S\u2010adenosylmethionine deficiency suppressed H3K27me3 and cell differentiation during neural tube development in mice"],"prefix":"10.1002","volume":"240","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4905-7053","authenticated-orcid":false,"given":"Li","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery and Liver Transplant Center The First Hospital of Shanxi Medical 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