{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:59:09Z","timestamp":1788274749133,"version":"build-2803163510"},"reference-count":86,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Shear stress, resulting from fluid flow, is a fundamental mechanical stimulus affecting various cellular functions. The epididymis, essential for sperm maturation, offers a compelling model to study the effects of shear stress on cellular behavior. This organ undergoes extensive proliferation and differentiation until puberty, achieving full functionality as spermatozoa commence their post\u2010testicular maturation. Although the mechanical tension exerted by testicular fluid is hypothesized to drive epithelial proliferation and differentiation, the precise mechanisms remain unclear. Here we assessed whether the responsiveness of the epididymal cells to shear stress depends on functional primary cilia by combining microfluidic strategies on immortalized epididymal cells, calcium signaling assays, and high\u2010throughput gene expression analysis. We identified 97 genes overexpressed in response to shear stress, including early growth response (Egr) 2\/3, cellular communication network factor (Ccn) 1\/2, and Fos proto\u2010oncogene (Fos). While shear stress triggered a rapid increase of intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , this response was abrogated following the impairment of primary ciliogenesis through pharmacological and siRNA approaches. Overall, our findings provide valuable insights into how mechanical forces influence the development of the male reproductive system, a requisite to sperm maturation.\n                  <\/jats:p>","DOI":"10.1002\/jcp.31475","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T08:55:04Z","timestamp":1730969704000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Shear stress effects on epididymal epithelial cell via primary cilia mechanosensory signaling"],"prefix":"10.1002","volume":"240","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1439-7666","authenticated-orcid":false,"given":"Sepideh","family":"Fakhari","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproduction, Centre de recherche en Reproduction, D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Faculty of Medicine Qu\u00e9bec City Quebec Canada"},{"name":"Centre de recherche du centre hospitalier universitaire de Qu\u00e9bec \u2010 Universit\u00e9 Laval Qu\u00e9bec City Quebec Canada"},{"name":"Department of Chemistry Faculty of Science and Engineering Qu\u00e9bec City Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Campolina\u2010Silva","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproduction, Centre de recherche en Reproduction, D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Faculty of Medicine Qu\u00e9bec City Quebec Canada"},{"name":"Centre de recherche du centre hospitalier universitaire de Qu\u00e9bec \u2010 Universit\u00e9 Laval Qu\u00e9bec City Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farnaz","family":"Asayesh","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproduction, Centre de recherche en Reproduction, D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Faculty of Medicine Qu\u00e9bec City Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Girardet","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproduction, Centre de recherche en Reproduction, D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Faculty of Medicine Qu\u00e9bec City Quebec Canada"},{"name":"Centre de recherche du centre hospitalier universitaire de Qu\u00e9bec \u2010 Universit\u00e9 Laval Qu\u00e9bec City Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Pier","family":"Scott\u2010Boyer","sequence":"additional","affiliation":[{"name":"Proteomics Platform, Qu\u00e9bec Genomic Center, Universit\u00e9 Laval, CHU de Qu\u00e9bec Research Center (CHUL) Qu\u00e9bec City Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Droit","sequence":"additional","affiliation":[{"name":"Proteomics Platform, Qu\u00e9bec Genomic Center, Universit\u00e9 Laval, CHU de Qu\u00e9bec Research Center (CHUL) Qu\u00e9bec City Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Soulet","sequence":"additional","affiliation":[{"name":"Centre de recherche du centre hospitalier universitaire de Qu\u00e9bec \u2010 Universit\u00e9 Laval Qu\u00e9bec City Quebec Canada"},{"name":"Facult\u00e9 de pharmacie Universit\u00e9 Laval Qu\u00e9bec City Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse","family":"Greener","sequence":"additional","affiliation":[{"name":"Centre de recherche du centre hospitalier universitaire de Qu\u00e9bec \u2010 Universit\u00e9 Laval Qu\u00e9bec City Quebec Canada"},{"name":"Department of Chemistry Faculty of Science and Engineering Qu\u00e9bec City Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mence","family":"Belleann\u00e9e","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproduction, Centre de recherche en Reproduction, D\u00e9veloppement et Sant\u00e9 Interg\u00e9n\u00e9rationnelle Faculty of Medicine Qu\u00e9bec City Quebec Canada"},{"name":"Centre de recherche du centre hospitalier universitaire de Qu\u00e9bec \u2010 Universit\u00e9 Laval Qu\u00e9bec City Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.192765"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.099"},{"key":"e_1_2_8_4_1","unstructured":"Andrews S.FastQC: A quality control tool for high throughput sequence data. 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