{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:48Z","timestamp":1753869408533,"version":"3.41.2"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T00:00:00Z","timestamp":1734998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>MicroRNAs (miRNAs) are endogenous noncoding RNAs that produce a remarked effect on regulating posttranscriptional gene expression. Our previous study identified a decrease in the expression of oar\u2010miR\u2010411a\u20105p from umbilical plasma in intrauterine growth restriction (IUGR) Hu lambs subjected to maternal heat stress. In this study, we demonstrated that oar\u2010miR\u2010411a\u20105p could modulate skeletal muscle development. Overexpression of oar\u2010miR\u2010411a\u20105p significantly enhanced proliferation and differentiation in heat\u2010stressed Hu sheep myoblasts while suppressing apoptosis. Conversely, inhibition of oar\u2010miR\u2010411a\u20105p resulted in opposing effects. Subsequently, RNAhybrid analysis revealed targeted sites between oar\u2010miR\u2010411a\u20105p and the 3\u2032 untranslated region (UTR) of <jats:italic>SMAD2<\/jats:italic>. This suggested that <jats:italic>SMAD2<\/jats:italic> is a direct target gene of oar\u2010miR\u2010411a\u20105p, as its expression was negatively modulated by oar\u2010miR\u2010411a\u20105p, a finding corroborated by dual\u2010luciferase assay and RT\u2010qPCR. Furthermore, co\u2010transfection of oar\u2010miR\u2010411a\u20105p and SMAD2 into Hu sheep myoblasts indicated that oar\u2010miR\u2010411a\u20105p modulated heat\u2010stressed myoblast growth by targeting <jats:italic>SMAD2<\/jats:italic>. In conclusion, these findings elucidate the function of oar\u2010miR\u2010411a\u20105p in promoting the development of heat\u2010stressed Hu sheep myoblasts, thereby enhancing our understanding of how miRNAs influence skeletal muscle growth in heat\u2010stressed Hu sheep.<\/jats:p>","DOI":"10.1002\/jcp.31515","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T11:23:21Z","timestamp":1735039401000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["oar\u2010miR\u2010411a\u20105p Promotes Proliferation and Differentiation in Hu Sheep Myoblasts Under Heat Stress by Targeting <i>SMAD2<\/i>"],"prefix":"10.1002","volume":"240","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6076-7102","authenticated-orcid":false,"given":"Jiawei","family":"Lu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University Nanjing 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