{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:33:41Z","timestamp":1790422421507,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ghrelin is a peptide hormone primarily produced by ghrelin cells in the stomach, playing a vital role in the regulation of eating behavior. Adenyl cyclase 8 (ADCY8), a key downstream signaling factor of G protein\u2010coupled receptors, is essential for maintaining energy homeostasis by modulating levels of cyclic adenosine monophosphate (cAMP). Nevertheless, how ADCY8 modulates ghrelin levels and affects food intake is not well understood. Our findings demonstrated that\n                    <jats:italic>Adcy8<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice exhibited elevated levels of ghrelin and increased food consumption under both normal and high\u2010fat diet conditions. These changes were associated with a reduction in the activity of the cAMP\u2010PKA\u2010mTOR signaling pathway within the gastric mucosa. The administration of the ghrelin receptor antagonist\n                    <jats:sc>d<\/jats:sc>\n                    \u2010Lys\u20103\u2010GH\u2010releasing peptide\u20106 significantly decreased calorie intake in both wild\u2010type and\n                    <jats:italic>Adcy8<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice. Furthermore, forskolin was shown to inhibit ghrelin and calorie intake in normal mice, an effect that was absent in\n                    <jats:italic>Adcy8<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice. Treatment with forskolin or overexpression of\n                    <jats:italic>Adcy8<\/jats:italic>\n                    in both primary ghrelin\u2010producing cells and mHypoE\u201042 cells resulted in decreased ghrelin levels, accompanied by activation of the cAMP\u2010PKA\u2010mTOR signaling pathway. Conversely, the use of the inhibitor SQ22536 or knockdown of\n                    <jats:italic>Adcy8<\/jats:italic>\n                    produced opposing effects. In conclusion, gastric ADCY8 regulates the expression and secretion of ghrelin via the cAMP\u2010PKA\u2010mTOR signaling pathway, thereby influencing food intake.\n                  <\/jats:p>","DOI":"10.1002\/jcp.70087","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T12:52:49Z","timestamp":1756385569000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Regulation of Ghrelin Production and Food Intake by Gastric Adenylyl Cyclase Type 8"],"prefix":"10.1002","volume":"240","author":[{"given":"Shaohong","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Medicine Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8841-3237","authenticated-orcid":false,"given":"Handan","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5730-1694","authenticated-orcid":false,"given":"Ruili","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Henan Provincial People's Hospital People's Hospital of Zhengzhou University, People's Hospital of Henan University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Medicine, Medical Experimental Center Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luyang","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geyang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine Jinan University Guangzhou China"},{"name":"Key Laboratory of Viral Pathogenesis &amp; Infection Prevention and Control (Jinan University), Ministry of Education Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","DOI":"10.3390\/biology9090244","article-title":"Increased Plasma Levels of Adenylate Cyclase 8 and cAMP are Associated With Obesity and Type 2 Diabetes: Results From a Cross\u2010Sectional Study","volume":"9","author":"Abdel\u2010Halim S. 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