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Macrophages and astrocytes play important roles in the inflammatory response. Tip cells, a critical endothelial sub\u2010population, play pivotal roles in post\u2010injury vascular regeneration. Nevertheless, their characteristics in SCI remain poorly documented. This study based on single cell RNA sequencing (scRNA\u2010seq) and in vitro experiment, investigates the effects of tip cells on astrocytes and macrophages. For astrocytes, tip cells can recruit astrocytes to migrant, contribute to the formation of fence\u2010like structure of astrocytes, finally inhibit the diffusion of inflammation via the Angptl4\u2010Sdc4 ligand\u2010receptor pathway. For macrophages, similarly through the Angptl4\u2010Sdc4 ligand\u2010receptor pathway, tip cells can promote macrophages to polarize more toward the M2 phenotype and inhibit their polarization toward M1 phenotype, thus alleviate the inflammatory response. Tip cells after SCI exhibit conserved ribosomal protein expression, implicating ribosome\u2010dependent signaling in their function. These finding highlight the critical role of tip cells in microenvironment after SCI, offering a potential treatment target for SCI.<\/jats:p>","DOI":"10.1002\/jcp.70088","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T11:39:10Z","timestamp":1756813150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Single\u2010Cell Sequencing\u2010Based Exploration of the Role of Tip Cells on Astrocytes and Macrophages After Spinal Cord Injury"],"prefix":"10.1002","volume":"240","author":[{"given":"Xiaolin","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Spine Surgery, The First Affiliated Hospital Sun Yat\u2010sen University Guangzhou Guangdong Province China"},{"name":"Guangdong Province Key Laboratory of Orthopedics and Traumatology Sun Yat\u2010sen University Guangzhou Guangdong Province China"},{"name":"Division of Spine Surgery, Department of Orthopedic 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